Headspace gas chromatography to determine human low density lipoprotein oxidation

E. N. Frankel, J. Bruce German, Paul A. Davis

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.

Original languageEnglish (US)
Pages (from-to)1047-1051
Number of pages5
JournalLipids
Volume27
Issue number12
DOIs
StatePublished - Dec 1992

Fingerprint

low density lipoprotein
Unsaturated Fatty Acids
LDL Lipoproteins
headspace analysis
Gas chromatography
Gas Chromatography
polyunsaturated fatty acids
gas chromatography
oxidation
Oxidation
peroxidation
omega-6 fatty acids
Antioxidants
Tocopherols
Omega-3 Fatty Acids
Cell membranes
Vitamin E
antioxidants
Liver
Rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Organic Chemistry
  • Cell Biology

Cite this

Headspace gas chromatography to determine human low density lipoprotein oxidation. / Frankel, E. N.; German, J. Bruce; Davis, Paul A.

In: Lipids, Vol. 27, No. 12, 12.1992, p. 1047-1051.

Research output: Contribution to journalArticle

Frankel, E. N. ; German, J. Bruce ; Davis, Paul A. / Headspace gas chromatography to determine human low density lipoprotein oxidation. In: Lipids. 1992 ; Vol. 27, No. 12. pp. 1047-1051.
@article{36c69dadfda4471a9acc516c820e8f72,
title = "Headspace gas chromatography to determine human low density lipoprotein oxidation",
abstract = "We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.",
author = "Frankel, {E. N.} and German, {J. Bruce} and Davis, {Paul A.}",
year = "1992",
month = "12",
doi = "10.1007/BF02535586",
language = "English (US)",
volume = "27",
pages = "1047--1051",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "12",

}

TY - JOUR

T1 - Headspace gas chromatography to determine human low density lipoprotein oxidation

AU - Frankel, E. N.

AU - German, J. Bruce

AU - Davis, Paul A.

PY - 1992/12

Y1 - 1992/12

N2 - We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.

AB - We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.

UR - http://www.scopus.com/inward/record.url?scp=0027096499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027096499&partnerID=8YFLogxK

U2 - 10.1007/BF02535586

DO - 10.1007/BF02535586

M3 - Article

C2 - 1487951

AN - SCOPUS:0027096499

VL - 27

SP - 1047

EP - 1051

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 12

ER -