Abstract
We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1047-1051 |
| Number of pages | 5 |
| Journal | Lipids |
| Volume | 27 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1992 |
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ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Food Science
- Organic Chemistry
- Cell Biology
Cite this
Headspace gas chromatography to determine human low density lipoprotein oxidation. / Frankel, E. N.; German, J. Bruce; Davis, Paul A.
In: Lipids, Vol. 27, No. 12, 12.1992, p. 1047-1051.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Headspace gas chromatography to determine human low density lipoprotein oxidation
AU - Frankel, E. N.
AU - German, J. Bruce
AU - Davis, Paul A.
PY - 1992/12
Y1 - 1992/12
N2 - We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.
AB - We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.
UR - http://www.scopus.com/inward/record.url?scp=0027096499&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027096499&partnerID=8YFLogxK
U2 - 10.1007/BF02535586
DO - 10.1007/BF02535586
M3 - Article
C2 - 1487951
AN - SCOPUS:0027096499
VL - 27
SP - 1047
EP - 1051
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 12
ER -