Abstract
We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P<0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to α-tocopherol, other endogenous antioxidants and factors may contribute, to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1047-1051 |
| Number of pages | 5 |
| Journal | Lipids |
| Volume | 27 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1992 |
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Food Science
- Organic Chemistry
- Cell Biology