Harvey-ras mediated neoplastic development in the mouse mammary gland

R. Strange, E. Aguilar-Cordova, L. J T Young, H. T. Billy, Satya Dandekar, Robert Cardiff

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The role of a Havey-ras oncogene in mammary epithelial neoplasia was examined by infecting primary cultures of normal mouse mammary epithelial cells with either the Harvey murine sarcoma virus (ψ2HaMSV) alone or with HaMSV plus a helper virus. The biological effects of expression of the Ha-ras oncogene were determined by transplanting the infected cells into gland-cleared mammary fat pads of virgin Balb/c mice. Expression of the Ha-ras oncogene was correlated with the developoment of mammary epithelial neoplasms. Cells infected with replication-defective HaMSV alone formed dysplastic, non-invasive mammary outgrowths. Cells infected with HaMSV plus a helper virus developed poorly-differentiated, invasive mammary epithelial tumors. Uninfected cells and cells infected with only the helper virus formed normal mammary trees. Expression of the mutant viral Ha-ras p21 was detected in dysplastic outgrowths and tumors but not in normal mammary outgrowths. Use of this transgenic organ system to genetically alter epithelium of the mouse mammary gland has permitted correlation of expression of a Ha-ras oncogene with development of mouse mammary neoplasia.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalOncogene
Volume4
Issue number3
StatePublished - 1989

Fingerprint

Human Mammary Glands
Breast
ras Genes
Helper Viruses
Harvey murine sarcoma virus
Animal Mammary Neoplasms
Proto-Oncogene Proteins p21(ras)
Neoplasms
Adipose Tissue
Epithelium
Epithelial Cells
Breast Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Strange, R., Aguilar-Cordova, E., Young, L. J. T., Billy, H. T., Dandekar, S., & Cardiff, R. (1989). Harvey-ras mediated neoplastic development in the mouse mammary gland. Oncogene, 4(3), 309-315.

Harvey-ras mediated neoplastic development in the mouse mammary gland. / Strange, R.; Aguilar-Cordova, E.; Young, L. J T; Billy, H. T.; Dandekar, Satya; Cardiff, Robert.

In: Oncogene, Vol. 4, No. 3, 1989, p. 309-315.

Research output: Contribution to journalArticle

Strange, R, Aguilar-Cordova, E, Young, LJT, Billy, HT, Dandekar, S & Cardiff, R 1989, 'Harvey-ras mediated neoplastic development in the mouse mammary gland', Oncogene, vol. 4, no. 3, pp. 309-315.
Strange R, Aguilar-Cordova E, Young LJT, Billy HT, Dandekar S, Cardiff R. Harvey-ras mediated neoplastic development in the mouse mammary gland. Oncogene. 1989;4(3):309-315.
Strange, R. ; Aguilar-Cordova, E. ; Young, L. J T ; Billy, H. T. ; Dandekar, Satya ; Cardiff, Robert. / Harvey-ras mediated neoplastic development in the mouse mammary gland. In: Oncogene. 1989 ; Vol. 4, No. 3. pp. 309-315.
@article{0139942b420c455eaa8cbc642f8df3b6,
title = "Harvey-ras mediated neoplastic development in the mouse mammary gland",
abstract = "The role of a Havey-ras oncogene in mammary epithelial neoplasia was examined by infecting primary cultures of normal mouse mammary epithelial cells with either the Harvey murine sarcoma virus (ψ2HaMSV) alone or with HaMSV plus a helper virus. The biological effects of expression of the Ha-ras oncogene were determined by transplanting the infected cells into gland-cleared mammary fat pads of virgin Balb/c mice. Expression of the Ha-ras oncogene was correlated with the developoment of mammary epithelial neoplasms. Cells infected with replication-defective HaMSV alone formed dysplastic, non-invasive mammary outgrowths. Cells infected with HaMSV plus a helper virus developed poorly-differentiated, invasive mammary epithelial tumors. Uninfected cells and cells infected with only the helper virus formed normal mammary trees. Expression of the mutant viral Ha-ras p21 was detected in dysplastic outgrowths and tumors but not in normal mammary outgrowths. Use of this transgenic organ system to genetically alter epithelium of the mouse mammary gland has permitted correlation of expression of a Ha-ras oncogene with development of mouse mammary neoplasia.",
author = "R. Strange and E. Aguilar-Cordova and Young, {L. J T} and Billy, {H. T.} and Satya Dandekar and Robert Cardiff",
year = "1989",
language = "English (US)",
volume = "4",
pages = "309--315",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Harvey-ras mediated neoplastic development in the mouse mammary gland

AU - Strange, R.

AU - Aguilar-Cordova, E.

AU - Young, L. J T

AU - Billy, H. T.

AU - Dandekar, Satya

AU - Cardiff, Robert

PY - 1989

Y1 - 1989

N2 - The role of a Havey-ras oncogene in mammary epithelial neoplasia was examined by infecting primary cultures of normal mouse mammary epithelial cells with either the Harvey murine sarcoma virus (ψ2HaMSV) alone or with HaMSV plus a helper virus. The biological effects of expression of the Ha-ras oncogene were determined by transplanting the infected cells into gland-cleared mammary fat pads of virgin Balb/c mice. Expression of the Ha-ras oncogene was correlated with the developoment of mammary epithelial neoplasms. Cells infected with replication-defective HaMSV alone formed dysplastic, non-invasive mammary outgrowths. Cells infected with HaMSV plus a helper virus developed poorly-differentiated, invasive mammary epithelial tumors. Uninfected cells and cells infected with only the helper virus formed normal mammary trees. Expression of the mutant viral Ha-ras p21 was detected in dysplastic outgrowths and tumors but not in normal mammary outgrowths. Use of this transgenic organ system to genetically alter epithelium of the mouse mammary gland has permitted correlation of expression of a Ha-ras oncogene with development of mouse mammary neoplasia.

AB - The role of a Havey-ras oncogene in mammary epithelial neoplasia was examined by infecting primary cultures of normal mouse mammary epithelial cells with either the Harvey murine sarcoma virus (ψ2HaMSV) alone or with HaMSV plus a helper virus. The biological effects of expression of the Ha-ras oncogene were determined by transplanting the infected cells into gland-cleared mammary fat pads of virgin Balb/c mice. Expression of the Ha-ras oncogene was correlated with the developoment of mammary epithelial neoplasms. Cells infected with replication-defective HaMSV alone formed dysplastic, non-invasive mammary outgrowths. Cells infected with HaMSV plus a helper virus developed poorly-differentiated, invasive mammary epithelial tumors. Uninfected cells and cells infected with only the helper virus formed normal mammary trees. Expression of the mutant viral Ha-ras p21 was detected in dysplastic outgrowths and tumors but not in normal mammary outgrowths. Use of this transgenic organ system to genetically alter epithelium of the mouse mammary gland has permitted correlation of expression of a Ha-ras oncogene with development of mouse mammary neoplasia.

UR - http://www.scopus.com/inward/record.url?scp=0024601448&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024601448&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 309

EP - 315

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 3

ER -