Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

Carolyn Bolden-Watson; Michael A. Watson; Karl D Murray; Paul J. Isackson; Elliott Richelson

Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

Carolyn Bolden-Watson, Michael A. Watson, Karl D Murray, Paul J. Isackson, Elliott Richelson

Research output: Contribution to journal › Article › peer-review

Abstract

We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.

Original language	English (US)
Pages (from-to)	1141-1143
Number of pages	3
Journal	Journal of Neurochemistry
Volume	61
Issue number	3
State	Published - Sep 1993
Externally published	Yes

Keywords

Atypical neuroleptics
Extrapyramidal side effects
In situ hybridization histochemistry

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra'. Together they form a unique fingerprint.

Cite this

@article{95a9b7e0d3bf472997305ef5d7c6a0f2,

title = "Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra",

abstract = "We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.",

keywords = "Atypical neuroleptics, Extrapyramidal side effects, In situ hybridization histochemistry",

author = "Carolyn Bolden-Watson and Watson, {Michael A.} and Murray, {Karl D} and Isackson, {Paul J.} and Elliott Richelson",

year = "1993",

month = sep,

language = "English (US)",

volume = "61",

pages = "1141--1143",

journal = "Journal of Neurochemistry",

issn = "0022-3042",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

AU - Bolden-Watson, Carolyn

AU - Watson, Michael A.

AU - Murray, Karl D

AU - Isackson, Paul J.

AU - Richelson, Elliott

PY - 1993/9

Y1 - 1993/9

N2 - We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.

AB - We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.

KW - Atypical neuroleptics

KW - Extrapyramidal side effects

KW - In situ hybridization histochemistry

UR - http://www.scopus.com/inward/record.url?scp=0027261520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027261520&partnerID=8YFLogxK

M3 - Article

C2 - 8395560

AN - SCOPUS:0027261520

VL - 61

SP - 1141

EP - 1143

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 3

ER -

Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Cite this