Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

Carolyn Bolden-Watson; Michael A. Watson; Karl D Murray; Paul J. Isackson; Elliott Richelson

Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

Carolyn Bolden-Watson, Michael A. Watson, Karl D Murray, Paul J. Isackson, Elliott Richelson

Research output: Contribution to journal › Article › peer-review

Abstract

We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.

Original language	English (US)
Pages (from-to)	1141-1143
Number of pages	3
Journal	Journal of Neurochemistry
Volume	61
Issue number	3
State	Published - Sep 1993
Externally published	Yes

Keywords

Atypical neuroleptics
Extrapyramidal side effects
In situ hybridization histochemistry

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

Cite this

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title = "Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra",

abstract = "We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.",

keywords = "Atypical neuroleptics, Extrapyramidal side effects, In situ hybridization histochemistry",

author = "Carolyn Bolden-Watson and Watson, {Michael A.} and Murray, {Karl D} and Isackson, {Paul J.} and Elliott Richelson",

year = "1993",

month = sep,

language = "English (US)",

volume = "61",

pages = "1141--1143",

journal = "Journal of Neurochemistry",

issn = "0022-3042",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

AU - Bolden-Watson, Carolyn

AU - Watson, Michael A.

AU - Murray, Karl D

AU - Isackson, Paul J.

AU - Richelson, Elliott

PY - 1993/9

Y1 - 1993/9

N2 - We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.

AB - We examined the effects of chronic (2 weeks) treatment with a typical neuroleptic, haloperidol (1 mg/kg, s.c.), and an atypical neuroleptic, clozapine (20 mg/kg, s.c.), on neurotensin receptor (NTR) mRNA levels by in situ hybridization histochemistry. Quantitative OD analysis showed haloperidol-induced NTR mRNA levels in the substantia nigra/ventral tegmental area (SN/ VTA) 110% over control levels (significant difference from the control, p < 0.05). In contrast, the same analysis applied to the sections from clozapine-treated animals showed no significant change in NTR mRNA levels compared with matched control sections (p > 0.05). Thus, chronic treatment with haloperidol but not clozapine resulted in elevated levels of NTR mRNA within the SN/VTA. These results suggest that the high incidence of extrapyramidal side effects of typical neuroleptics could result from changes in NTR expression in the SN/VTA.

KW - Atypical neuroleptics

KW - Extrapyramidal side effects

KW - In situ hybridization histochemistry

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JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

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ER -

Haloperidol but not clozapine increases neurotensin receptor mRNA levels in rat substantia nigra

Abstract

Keywords

ASJC Scopus subject areas

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