Halide transport in Xenopus oocytes

Y. Katayama, Jonathan Widdicombe

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

1. Radioisotopes and intracellular microelectrodes were used to characterize the permeability of Xenopus oocytes to chloride and other halides. 2. Uptake of 36Cl had a half-time for equilibration of ~ 3 h, with an initial rate of Cl- entry corresponding to a permeability coefficient of 3.9 x 10-7 cm/s, and an equilibrium uptake of 36Cl of 33 mM. 3. Replacement of bathing Na+ by K+ depolarized the oocytes from -46 to -7 mV and stimulated influx approximately 3-fold. 4. Influx was linearly dependent on bathing [Cl-] and was temperature dependent with an activation energy of 46 kJ/mol. Influx of 125I or 36Cl was not affected by the presence of equal concentrations of other halides or thiocyanate. These results are consistent with a channel-mediated entry mechanism. 5. Diphenylamine-2-carboxylate (DPAC) and 9-anthracene carboxylate (9-AC), blockers of Cl- channels in other cells, inhibited Cl- entry with dissociation constants (K(d)s) of ~ 5 x 10-4 and ~ 10-3 M, respectively. Inhibitors of Cl--HCO3 - exchange or Na+--K+--2Cl- co-transport did not affect Cl- influx. 6. Attempts to lower or raise intracellular Ca2+ with BAPTA or A23187, respectively, were also without effect on Cl- influx. 7. The halide selectivity sequence determined with isotopes was I- (3.2) > Br- (1.3) > Cl- (1.0). However, DPAC inhibited almost all of the 36Cl influx but only a small fraction of 125I influx. 8. Replacement of bathing Cl- by I- or Br- resulted in hyperpolarizations, from which the same selectivity sequence was determined. 9. Replacement of bathing Cl- by gluconate caused a marked depolarization, which was inhibited by DPAC and, less potently, by 9-AC.

Original languageEnglish (US)
Pages (from-to)587-599
Number of pages13
JournalJournal of Physiology
Volume443
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Halide transport in Xenopus oocytes'. Together they form a unique fingerprint.

Cite this