Haemolymph juvenile hormone esterase activity in synchronous last instar larvae of the cabbage looper, Trichoplusia ni

Thomas C. Sparks, William S. Willis, Harry H. Shorey, Bruce D. Hammock

Research output: Contribution to journalArticle

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Abstract

Weight and time of moult during the last instar of the cabbage looper (Trichoplusia ni) were examined and used to select last instar larvae that had similar rates of development. Haemolymph protein content and titres of haemolymph esterases hydrolyzing juvenile hormone I, juvenile hormone III, and α-naphthyl acetate were monitored during the last instar using these closely timed larvae. Juvenile hormone I and juvenile hormone III esterase profiles were very similar and differed markedly from the α-naphthyl acetate esterase and protein content profiles. Two major peaks of juvenile hormone esterase activity were observed, one before ecdysone release and the other just prior to pupal ecdysis. Juvenile hormone I was hydrolyzed 15 times faster than juvenile hormone III when assayed at 5 × 10-6 M.

Original languageEnglish (US)
Pages (from-to)125-132
Number of pages8
JournalJournal of Insect Physiology
Volume25
Issue number2
DOIs
StatePublished - 1979

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Keywords

  • degradation
  • development
  • esterase
  • juvenile hormone
  • Noctuidae
  • Trichoplusia ni

ASJC Scopus subject areas

  • Insect Science
  • Physiology

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