Haemolymph juvenile hormone esterase activity in synchronous last instar larvae of the cabbage looper, Trichoplusia ni

Thomas C. Sparks, William S. Willis, Harry H. Shorey, Bruce D. Hammock

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Weight and time of moult during the last instar of the cabbage looper (Trichoplusia ni) were examined and used to select last instar larvae that had similar rates of development. Haemolymph protein content and titres of haemolymph esterases hydrolyzing juvenile hormone I, juvenile hormone III, and α-naphthyl acetate were monitored during the last instar using these closely timed larvae. Juvenile hormone I and juvenile hormone III esterase profiles were very similar and differed markedly from the α-naphthyl acetate esterase and protein content profiles. Two major peaks of juvenile hormone esterase activity were observed, one before ecdysone release and the other just prior to pupal ecdysis. Juvenile hormone I was hydrolyzed 15 times faster than juvenile hormone III when assayed at 5 × 10-6 M.

Original languageEnglish (US)
Pages (from-to)125-132
Number of pages8
JournalJournal of Insect Physiology
Volume25
Issue number2
DOIs
StatePublished - 1979

Fingerprint

juvenile hormone esterase
Trichoplusia ni
Hemolymph
Brassica
juvenile hormones
hemolymph
Larva
instars
larvae
esterases
Acetylesterase
Ecdysone
Molting
Esterases
Proteins
acetates
protein content
Weights and Measures
ecdysone
ecdysis

Keywords

  • degradation
  • development
  • esterase
  • juvenile hormone
  • Noctuidae
  • Trichoplusia ni

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Haemolymph juvenile hormone esterase activity in synchronous last instar larvae of the cabbage looper, Trichoplusia ni. / Sparks, Thomas C.; Willis, William S.; Shorey, Harry H.; Hammock, Bruce D.

In: Journal of Insect Physiology, Vol. 25, No. 2, 1979, p. 125-132.

Research output: Contribution to journalArticle

Sparks, Thomas C. ; Willis, William S. ; Shorey, Harry H. ; Hammock, Bruce D. / Haemolymph juvenile hormone esterase activity in synchronous last instar larvae of the cabbage looper, Trichoplusia ni. In: Journal of Insect Physiology. 1979 ; Vol. 25, No. 2. pp. 125-132.
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