TY - JOUR
T1 - H. pylori α1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides
AU - Yu, Hai
AU - Li, Yanhong
AU - Wu, Zhigang
AU - Li, Lei
AU - Zeng, Jie
AU - Zhao, Chao
AU - Wu, Yijing
AU - Tasnima, Nova
AU - Wang, Jing
AU - Liu, Huaide
AU - Gadi, Madhusudhan Reddy
AU - Guan, Wanyi
AU - Wang, Peng G.
AU - Chen, Xi
PY - 2017
Y1 - 2017
N2 - Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).
AB - Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).
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U2 - 10.1039/c7cc05403c
DO - 10.1039/c7cc05403c
M3 - Article
C2 - 28936496
AN - SCOPUS:85030759972
VL - 53
SP - 11012
EP - 11015
JO - Chemical Communications
JF - Chemical Communications
SN - 1359-7345
IS - 80
ER -