H. pylori α1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides

Hai Yu; Yanhong Li; Zhigang Wu; Lei Li; Jie Zeng; Chao Zhao; Yijing Wu; Nova Tasnima; Jing Wang; Huaide Liu; Madhusudhan Reddy Gadi; Wanyi Guan; Peng G. Wang; Xi Chen

doi:10.1039/c7cc05403c

H. pylori α1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides

Hai Yu, Yanhong Li, Zhigang Wu, Lei Li, Jie Zeng, Chao Zhao, Yijing Wu, Nova Tasnima, Jing Wang, Huaide Liu, Madhusudhan Reddy Gadi, Wanyi Guan, Peng G. Wang, Xi Chen

Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L^-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).

Original language	English (US)
Pages (from-to)	11012-11015
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	80
DOIs	https://doi.org/10.1039/c7cc05403c
State	Published - 2017

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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H. pylori α1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides. / Yu, Hai; Li, Yanhong; Wu, Zhigang; Li, Lei; Zeng, Jie; Zhao, Chao; Wu, Yijing; Tasnima, Nova; Wang, Jing; Liu, Huaide; Gadi, Madhusudhan Reddy; Guan, Wanyi; Wang, Peng G.; Chen, Xi.

In: Chemical Communications, Vol. 53, No. 80, 2017, p. 11012-11015.

Research output: Contribution to journal › Article › peer-review

Yu, Hai ; Li, Yanhong ; Wu, Zhigang ; Li, Lei ; Zeng, Jie ; Zhao, Chao ; Wu, Yijing ; Tasnima, Nova ; Wang, Jing ; Liu, Huaide ; Gadi, Madhusudhan Reddy ; Guan, Wanyi ; Wang, Peng G. ; Chen, Xi. / H. pylori α1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides. In: Chemical Communications. 2017 ; Vol. 53, No. 80. pp. 11012-11015.

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abstract = "Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).",

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