Abstract
Objective A close relationship between gut microbiota and some chronic liver disorders has recently been described. Herein, we systematically performed a comparative analysis of the gut microbiome in primary biliary cholangitis (PBC) and healthy controls. Design We first conducted a cross-sectional study of 60 ursodeoxycholic acid (UDCA) treatment-naïve patients with PBC and 80 matched healthy controls. Second, an independent cohort composed of 19 treatment-naïve patients and 34 controls was used to validate the results. Finally, a prospective study was performed in a subgroup of 37 patients with PBC who underwent analysis before and after 6 months of UDCA treatment. Faecal samples were collected, and microbiomes were analysed by 16S ribosomal RNA gene sequencing. Results A significant reduction of within-individual microbial diversity was noted in PBC (p=0.03). A signature defined by decreased abundance of four genera and increased abundance of eight genera strongly correlated with PBC (area under curve=0.86, 0.84 in exploration and validation data, respectively). Notably, the abundance of six PBC-associated genera was reversed after 6 months of UDCA treatment. In particular, Faecalibacterium, enriched in controls, was further decreased in gp210-positive than gp210-negative patients (p=0.002). Of interest was the finding that the increased capacity for the inferred pathway, bacterial invasion of epithelial cells in PBC, highly correlated with the abundance of bacteria belonging to Enterobacteriaceae. Conclusions This study presents a comprehensive landscape of gut microbiota in PBC. Dysbiosis was found in the gut microbiome in PBC and partially relieved by UDCA. Our study suggests that gut microbiota is a potential therapeutic target and diagnostic biomarker for PBC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 534-571 |
| Number of pages | 38 |
| Journal | Gut |
| Volume | 67 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2018 |
Fingerprint
Keywords
- Intestinal Bacteria
- Primary Biliary Cirrhosis
ASJC Scopus subject areas
- Gastroenterology
Cite this
Gut microbial profile is altered in primary biliary cholangitis and partially restored after UDCA therapy. / Tang, Ruqi; Wei, Yiran; Li, Yanmei; Chen, Weihua; Chen, Haoyan; Wang, Qixia; Yang, Fan; Miao, Qi; Xiao, Xiao; Zhang, Haiyan; Lian, Min; Jiang, Xiang; Zhang, Jun; Cao, Qin; Fan, Zhuping; Wu, Maoying; Qiu, Dekai; Fang, Jing Yuan; Ansari, Aftab; Gershwin, M. Eric; Ma, Xiong.
In: Gut, Vol. 67, No. 3, 01.03.2018, p. 534-571.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gut microbial profile is altered in primary biliary cholangitis and partially restored after UDCA therapy
AU - Tang, Ruqi
AU - Wei, Yiran
AU - Li, Yanmei
AU - Chen, Weihua
AU - Chen, Haoyan
AU - Wang, Qixia
AU - Yang, Fan
AU - Miao, Qi
AU - Xiao, Xiao
AU - Zhang, Haiyan
AU - Lian, Min
AU - Jiang, Xiang
AU - Zhang, Jun
AU - Cao, Qin
AU - Fan, Zhuping
AU - Wu, Maoying
AU - Qiu, Dekai
AU - Fang, Jing Yuan
AU - Ansari, Aftab
AU - Gershwin, M. Eric
AU - Ma, Xiong
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Objective A close relationship between gut microbiota and some chronic liver disorders has recently been described. Herein, we systematically performed a comparative analysis of the gut microbiome in primary biliary cholangitis (PBC) and healthy controls. Design We first conducted a cross-sectional study of 60 ursodeoxycholic acid (UDCA) treatment-naïve patients with PBC and 80 matched healthy controls. Second, an independent cohort composed of 19 treatment-naïve patients and 34 controls was used to validate the results. Finally, a prospective study was performed in a subgroup of 37 patients with PBC who underwent analysis before and after 6 months of UDCA treatment. Faecal samples were collected, and microbiomes were analysed by 16S ribosomal RNA gene sequencing. Results A significant reduction of within-individual microbial diversity was noted in PBC (p=0.03). A signature defined by decreased abundance of four genera and increased abundance of eight genera strongly correlated with PBC (area under curve=0.86, 0.84 in exploration and validation data, respectively). Notably, the abundance of six PBC-associated genera was reversed after 6 months of UDCA treatment. In particular, Faecalibacterium, enriched in controls, was further decreased in gp210-positive than gp210-negative patients (p=0.002). Of interest was the finding that the increased capacity for the inferred pathway, bacterial invasion of epithelial cells in PBC, highly correlated with the abundance of bacteria belonging to Enterobacteriaceae. Conclusions This study presents a comprehensive landscape of gut microbiota in PBC. Dysbiosis was found in the gut microbiome in PBC and partially relieved by UDCA. Our study suggests that gut microbiota is a potential therapeutic target and diagnostic biomarker for PBC.
AB - Objective A close relationship between gut microbiota and some chronic liver disorders has recently been described. Herein, we systematically performed a comparative analysis of the gut microbiome in primary biliary cholangitis (PBC) and healthy controls. Design We first conducted a cross-sectional study of 60 ursodeoxycholic acid (UDCA) treatment-naïve patients with PBC and 80 matched healthy controls. Second, an independent cohort composed of 19 treatment-naïve patients and 34 controls was used to validate the results. Finally, a prospective study was performed in a subgroup of 37 patients with PBC who underwent analysis before and after 6 months of UDCA treatment. Faecal samples were collected, and microbiomes were analysed by 16S ribosomal RNA gene sequencing. Results A significant reduction of within-individual microbial diversity was noted in PBC (p=0.03). A signature defined by decreased abundance of four genera and increased abundance of eight genera strongly correlated with PBC (area under curve=0.86, 0.84 in exploration and validation data, respectively). Notably, the abundance of six PBC-associated genera was reversed after 6 months of UDCA treatment. In particular, Faecalibacterium, enriched in controls, was further decreased in gp210-positive than gp210-negative patients (p=0.002). Of interest was the finding that the increased capacity for the inferred pathway, bacterial invasion of epithelial cells in PBC, highly correlated with the abundance of bacteria belonging to Enterobacteriaceae. Conclusions This study presents a comprehensive landscape of gut microbiota in PBC. Dysbiosis was found in the gut microbiome in PBC and partially relieved by UDCA. Our study suggests that gut microbiota is a potential therapeutic target and diagnostic biomarker for PBC.
KW - Intestinal Bacteria
KW - Primary Biliary Cirrhosis
UR - http://www.scopus.com/inward/record.url?scp=85042885903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042885903&partnerID=8YFLogxK
U2 - 10.1136/gutjnl-2016-313332
DO - 10.1136/gutjnl-2016-313332
M3 - Article
C2 - 28213609
AN - SCOPUS:85042885903
VL - 67
SP - 534
EP - 571
JO - Gut
JF - Gut
SN - 0017-5749
IS - 3
ER -