Guided bone regeneration using injectable vascular endothelial growth factor delivery gel

Darnell Kaigler, Eduardo Silva, David J. Mooney

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Vascularization underlies the success of guided bone regeneration (GBR) procedures. This study evaluates the regenerative potential of GBR in combination with vascular endothelial growth factor (VEGF) delivery via an injectable hydrogel system. Methods: Critical-sized defects were created in rat calvariae, and GBR procedures were performed with a collagen membrane alone (control), or plus bolus delivery of VEGF, or plus application of VEGF-releasing hydrogels (VEGF-Alg). Four and 8 weeks after treatment, defect sites were evaluated with microcomputed tomographic and histomorphometric analyses for blood vessel and bone formation. Results: At 4 weeks, relative to the control condition, the bolus addition of VEGF did not affect blood vessel density within the defect site, yet the application of VEGF-Alg significantly (P <0.05) increased blood vessel density. Although there was no difference in bone regeneration at 4 weeks, at 8 weeks there was a significant (P <0.05) increase in bone regeneration in the VEGF-Alg-treated defects. Conclusions: These data demonstrate that the application of VEGF-Alg enhanced early angiogenesis, whereas at a later time point, it enhanced bone regeneration. Controlled delivery approaches of angiogenic growth factors used adjunctively with GBR may be a promising strategy for enhancing outcomes of GBR.

Original languageEnglish (US)
Pages (from-to)230-238
Number of pages9
JournalJournal of Periodontology
Volume84
Issue number2
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

Fingerprint

Bone Regeneration
Vascular Endothelial Growth Factor A
Gels
Injections
Blood Vessels
Hydrogels
Angiogenesis Inducing Agents
Hydrogel
Osteogenesis
Skull
Intercellular Signaling Peptides and Proteins
Collagen
Membranes

Keywords

  • Angiogenesis-inducing agents
  • Bioengineering
  • Bone and bones
  • Endothelial growth factors
  • Hydrogel
  • Regenerative medicine

ASJC Scopus subject areas

  • Periodontics

Cite this

Guided bone regeneration using injectable vascular endothelial growth factor delivery gel. / Kaigler, Darnell; Silva, Eduardo; Mooney, David J.

In: Journal of Periodontology, Vol. 84, No. 2, 01.02.2013, p. 230-238.

Research output: Contribution to journalArticle

@article{9f33714be99c442cbaaba9e5e7977aec,
title = "Guided bone regeneration using injectable vascular endothelial growth factor delivery gel",
abstract = "Background: Vascularization underlies the success of guided bone regeneration (GBR) procedures. This study evaluates the regenerative potential of GBR in combination with vascular endothelial growth factor (VEGF) delivery via an injectable hydrogel system. Methods: Critical-sized defects were created in rat calvariae, and GBR procedures were performed with a collagen membrane alone (control), or plus bolus delivery of VEGF, or plus application of VEGF-releasing hydrogels (VEGF-Alg). Four and 8 weeks after treatment, defect sites were evaluated with microcomputed tomographic and histomorphometric analyses for blood vessel and bone formation. Results: At 4 weeks, relative to the control condition, the bolus addition of VEGF did not affect blood vessel density within the defect site, yet the application of VEGF-Alg significantly (P <0.05) increased blood vessel density. Although there was no difference in bone regeneration at 4 weeks, at 8 weeks there was a significant (P <0.05) increase in bone regeneration in the VEGF-Alg-treated defects. Conclusions: These data demonstrate that the application of VEGF-Alg enhanced early angiogenesis, whereas at a later time point, it enhanced bone regeneration. Controlled delivery approaches of angiogenic growth factors used adjunctively with GBR may be a promising strategy for enhancing outcomes of GBR.",
keywords = "Angiogenesis-inducing agents, Bioengineering, Bone and bones, Endothelial growth factors, Hydrogel, Regenerative medicine",
author = "Darnell Kaigler and Eduardo Silva and Mooney, {David J.}",
year = "2013",
month = "2",
day = "1",
doi = "10.1902/jop.2012.110684",
language = "English (US)",
volume = "84",
pages = "230--238",
journal = "Journal of Periodontology",
issn = "0022-3492",
publisher = "American Academy of Periodontology",
number = "2",

}

TY - JOUR

T1 - Guided bone regeneration using injectable vascular endothelial growth factor delivery gel

AU - Kaigler, Darnell

AU - Silva, Eduardo

AU - Mooney, David J.

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Background: Vascularization underlies the success of guided bone regeneration (GBR) procedures. This study evaluates the regenerative potential of GBR in combination with vascular endothelial growth factor (VEGF) delivery via an injectable hydrogel system. Methods: Critical-sized defects were created in rat calvariae, and GBR procedures were performed with a collagen membrane alone (control), or plus bolus delivery of VEGF, or plus application of VEGF-releasing hydrogels (VEGF-Alg). Four and 8 weeks after treatment, defect sites were evaluated with microcomputed tomographic and histomorphometric analyses for blood vessel and bone formation. Results: At 4 weeks, relative to the control condition, the bolus addition of VEGF did not affect blood vessel density within the defect site, yet the application of VEGF-Alg significantly (P <0.05) increased blood vessel density. Although there was no difference in bone regeneration at 4 weeks, at 8 weeks there was a significant (P <0.05) increase in bone regeneration in the VEGF-Alg-treated defects. Conclusions: These data demonstrate that the application of VEGF-Alg enhanced early angiogenesis, whereas at a later time point, it enhanced bone regeneration. Controlled delivery approaches of angiogenic growth factors used adjunctively with GBR may be a promising strategy for enhancing outcomes of GBR.

AB - Background: Vascularization underlies the success of guided bone regeneration (GBR) procedures. This study evaluates the regenerative potential of GBR in combination with vascular endothelial growth factor (VEGF) delivery via an injectable hydrogel system. Methods: Critical-sized defects were created in rat calvariae, and GBR procedures were performed with a collagen membrane alone (control), or plus bolus delivery of VEGF, or plus application of VEGF-releasing hydrogels (VEGF-Alg). Four and 8 weeks after treatment, defect sites were evaluated with microcomputed tomographic and histomorphometric analyses for blood vessel and bone formation. Results: At 4 weeks, relative to the control condition, the bolus addition of VEGF did not affect blood vessel density within the defect site, yet the application of VEGF-Alg significantly (P <0.05) increased blood vessel density. Although there was no difference in bone regeneration at 4 weeks, at 8 weeks there was a significant (P <0.05) increase in bone regeneration in the VEGF-Alg-treated defects. Conclusions: These data demonstrate that the application of VEGF-Alg enhanced early angiogenesis, whereas at a later time point, it enhanced bone regeneration. Controlled delivery approaches of angiogenic growth factors used adjunctively with GBR may be a promising strategy for enhancing outcomes of GBR.

KW - Angiogenesis-inducing agents

KW - Bioengineering

KW - Bone and bones

KW - Endothelial growth factors

KW - Hydrogel

KW - Regenerative medicine

UR - http://www.scopus.com/inward/record.url?scp=84873549333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873549333&partnerID=8YFLogxK

U2 - 10.1902/jop.2012.110684

DO - 10.1902/jop.2012.110684

M3 - Article

C2 - 22668339

AN - SCOPUS:84873549333

VL - 84

SP - 230

EP - 238

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 2

ER -