Growth hormone enhances arachidonic acid metabolites in a growth hormone transgenic mouse

A. M. Oberbauer, J. B. German, J. D. Murray

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In a transgenic growth hormone (GH) mouse model, highly elevated GH increases overall growth and decreases adipose depots while low or moderate circulating GH enhances adipose deposition with differential effects on body growth. Using this model, the effects of low, moderate, and high chronic GH on fatty acid composition were determined for adipose and hepatic tissue and the metabolites of 20:4n-6 (arachidonic acid) were characterized to identify metabolic targets of action of elevated GH. The products of Δ-9 desaturase in hepatic, but not adipose, tissue were reduced in response to elevated GH. Proportional to the level of circulating GH, the products of Δ-5 and Δ-6 were increased in both adipose and hepatic tissue for the omega-6 lipids (e.g., 20:4n-6), while only the hepatic tissues showed an increase for omega-3 lipids (e.g., 22:6n-3). The eicosanoids, PGE2 and 12-HETE, were elevated with high GH but circulating thromboxane was not. Hepatic PTGS1 and 2 (COX1 and COX 2), SOD1, and FADS2 (Δ-6 desaturase) mRNAs were increased with elevated GH while FAS mRNA was reduced; SCD1 (stearoyl-coenzyme A desaturase) and SCD2 mRNA did not significantly differ. The present study showed that GH influences the net flux through various aspects of lipid metabolism and especially the desaturase metabolic processes. The combination of altered metabolism and tissue specificity suggest that the regulation of membrane composition and its effects on signaling pathways, including the production and actions of eicosanoids, can be mediated by the GH regulatory axis.

Original languageEnglish (US)
Pages (from-to)495-504
Number of pages10
JournalLipids
Volume46
Issue number6
DOIs
StatePublished - Jun 2011

Fingerprint

Metabolites
Arachidonic Acid
Transgenic Mice
Growth Hormone
Tissue
Liver
Adipose Tissue
Eicosanoids
Messenger RNA
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Lipids
Organ Specificity
Thromboxanes
Growth
Chemical analysis
Lipid Metabolism
Dinoprostone
Metabolism
Fatty Acids
Fluxes

Keywords

  • Arachidonic acid
  • Fatty acid composition
  • Growth hormone

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Organic Chemistry

Cite this

Growth hormone enhances arachidonic acid metabolites in a growth hormone transgenic mouse. / Oberbauer, A. M.; German, J. B.; Murray, J. D.

In: Lipids, Vol. 46, No. 6, 06.2011, p. 495-504.

Research output: Contribution to journalArticle

Oberbauer, A. M. ; German, J. B. ; Murray, J. D. / Growth hormone enhances arachidonic acid metabolites in a growth hormone transgenic mouse. In: Lipids. 2011 ; Vol. 46, No. 6. pp. 495-504.
@article{e7ae2846022f45fe86e8c7545c58fc30,
title = "Growth hormone enhances arachidonic acid metabolites in a growth hormone transgenic mouse",
abstract = "In a transgenic growth hormone (GH) mouse model, highly elevated GH increases overall growth and decreases adipose depots while low or moderate circulating GH enhances adipose deposition with differential effects on body growth. Using this model, the effects of low, moderate, and high chronic GH on fatty acid composition were determined for adipose and hepatic tissue and the metabolites of 20:4n-6 (arachidonic acid) were characterized to identify metabolic targets of action of elevated GH. The products of Δ-9 desaturase in hepatic, but not adipose, tissue were reduced in response to elevated GH. Proportional to the level of circulating GH, the products of Δ-5 and Δ-6 were increased in both adipose and hepatic tissue for the omega-6 lipids (e.g., 20:4n-6), while only the hepatic tissues showed an increase for omega-3 lipids (e.g., 22:6n-3). The eicosanoids, PGE2 and 12-HETE, were elevated with high GH but circulating thromboxane was not. Hepatic PTGS1 and 2 (COX1 and COX 2), SOD1, and FADS2 (Δ-6 desaturase) mRNAs were increased with elevated GH while FAS mRNA was reduced; SCD1 (stearoyl-coenzyme A desaturase) and SCD2 mRNA did not significantly differ. The present study showed that GH influences the net flux through various aspects of lipid metabolism and especially the desaturase metabolic processes. The combination of altered metabolism and tissue specificity suggest that the regulation of membrane composition and its effects on signaling pathways, including the production and actions of eicosanoids, can be mediated by the GH regulatory axis.",
keywords = "Arachidonic acid, Fatty acid composition, Growth hormone",
author = "Oberbauer, {A. M.} and German, {J. B.} and Murray, {J. D.}",
year = "2011",
month = "6",
doi = "10.1007/s11745-011-3548-y",
language = "English (US)",
volume = "46",
pages = "495--504",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Growth hormone enhances arachidonic acid metabolites in a growth hormone transgenic mouse

AU - Oberbauer, A. M.

AU - German, J. B.

AU - Murray, J. D.

PY - 2011/6

Y1 - 2011/6

N2 - In a transgenic growth hormone (GH) mouse model, highly elevated GH increases overall growth and decreases adipose depots while low or moderate circulating GH enhances adipose deposition with differential effects on body growth. Using this model, the effects of low, moderate, and high chronic GH on fatty acid composition were determined for adipose and hepatic tissue and the metabolites of 20:4n-6 (arachidonic acid) were characterized to identify metabolic targets of action of elevated GH. The products of Δ-9 desaturase in hepatic, but not adipose, tissue were reduced in response to elevated GH. Proportional to the level of circulating GH, the products of Δ-5 and Δ-6 were increased in both adipose and hepatic tissue for the omega-6 lipids (e.g., 20:4n-6), while only the hepatic tissues showed an increase for omega-3 lipids (e.g., 22:6n-3). The eicosanoids, PGE2 and 12-HETE, were elevated with high GH but circulating thromboxane was not. Hepatic PTGS1 and 2 (COX1 and COX 2), SOD1, and FADS2 (Δ-6 desaturase) mRNAs were increased with elevated GH while FAS mRNA was reduced; SCD1 (stearoyl-coenzyme A desaturase) and SCD2 mRNA did not significantly differ. The present study showed that GH influences the net flux through various aspects of lipid metabolism and especially the desaturase metabolic processes. The combination of altered metabolism and tissue specificity suggest that the regulation of membrane composition and its effects on signaling pathways, including the production and actions of eicosanoids, can be mediated by the GH regulatory axis.

AB - In a transgenic growth hormone (GH) mouse model, highly elevated GH increases overall growth and decreases adipose depots while low or moderate circulating GH enhances adipose deposition with differential effects on body growth. Using this model, the effects of low, moderate, and high chronic GH on fatty acid composition were determined for adipose and hepatic tissue and the metabolites of 20:4n-6 (arachidonic acid) were characterized to identify metabolic targets of action of elevated GH. The products of Δ-9 desaturase in hepatic, but not adipose, tissue were reduced in response to elevated GH. Proportional to the level of circulating GH, the products of Δ-5 and Δ-6 were increased in both adipose and hepatic tissue for the omega-6 lipids (e.g., 20:4n-6), while only the hepatic tissues showed an increase for omega-3 lipids (e.g., 22:6n-3). The eicosanoids, PGE2 and 12-HETE, were elevated with high GH but circulating thromboxane was not. Hepatic PTGS1 and 2 (COX1 and COX 2), SOD1, and FADS2 (Δ-6 desaturase) mRNAs were increased with elevated GH while FAS mRNA was reduced; SCD1 (stearoyl-coenzyme A desaturase) and SCD2 mRNA did not significantly differ. The present study showed that GH influences the net flux through various aspects of lipid metabolism and especially the desaturase metabolic processes. The combination of altered metabolism and tissue specificity suggest that the regulation of membrane composition and its effects on signaling pathways, including the production and actions of eicosanoids, can be mediated by the GH regulatory axis.

KW - Arachidonic acid

KW - Fatty acid composition

KW - Growth hormone

UR - http://www.scopus.com/inward/record.url?scp=79955680245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955680245&partnerID=8YFLogxK

U2 - 10.1007/s11745-011-3548-y

DO - 10.1007/s11745-011-3548-y

M3 - Article

C2 - 21442273

AN - SCOPUS:79955680245

VL - 46

SP - 495

EP - 504

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 6

ER -