Abstract
Purpose: The objective of this study was to use a nonhuman primate model to examine the effects of growth hormone (GH) on oocyte in vitro maturation (IVM). Methods: Immunocytochemistry confirmed the presence of GH receptors in rhesus cumulus oocyte complexes and the cytoplasm of embryonic blastomeres. Recombinant human GH (r-hGH) was added to IVM medium and cumulus expansion, nuclear maturation, cytoplasmic maturation and embryo development were analyzed. Results: Cumulus expansion was highest in the presence of 1 and 10 ng/ml r-hGH. The addition of r-hGH during IVM increased the percentage of embryos progressing to at least the 9-16 cell stage. In a separate study, 100 ng/ml r-hGH was supplemented to IVM and embryo culture medium and no effect was observed. Conclusions: The presence of GH receptors along with increased cumulus expansion and embryos progressing to the 9-16 cell stage supports the hypothesis that r-hGH may be involved in oocyte maturation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-158 |
| Number of pages | 14 |
| Journal | Journal of Assisted Reproduction and Genetics |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2008 |
Fingerprint
Keywords
- Cumulus cells
- Growth hormone
- Non-human primate
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Developmental Biology
- Genetics
- Reproductive Medicine
Cite this
Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development. / Nyholt De Prada, Jenna K.; VandeVoort, Catherine A.
In: Journal of Assisted Reproduction and Genetics, Vol. 25, No. 4, 04.2008, p. 145-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development
AU - Nyholt De Prada, Jenna K.
AU - VandeVoort, Catherine A.
PY - 2008/4
Y1 - 2008/4
N2 - Purpose: The objective of this study was to use a nonhuman primate model to examine the effects of growth hormone (GH) on oocyte in vitro maturation (IVM). Methods: Immunocytochemistry confirmed the presence of GH receptors in rhesus cumulus oocyte complexes and the cytoplasm of embryonic blastomeres. Recombinant human GH (r-hGH) was added to IVM medium and cumulus expansion, nuclear maturation, cytoplasmic maturation and embryo development were analyzed. Results: Cumulus expansion was highest in the presence of 1 and 10 ng/ml r-hGH. The addition of r-hGH during IVM increased the percentage of embryos progressing to at least the 9-16 cell stage. In a separate study, 100 ng/ml r-hGH was supplemented to IVM and embryo culture medium and no effect was observed. Conclusions: The presence of GH receptors along with increased cumulus expansion and embryos progressing to the 9-16 cell stage supports the hypothesis that r-hGH may be involved in oocyte maturation.
AB - Purpose: The objective of this study was to use a nonhuman primate model to examine the effects of growth hormone (GH) on oocyte in vitro maturation (IVM). Methods: Immunocytochemistry confirmed the presence of GH receptors in rhesus cumulus oocyte complexes and the cytoplasm of embryonic blastomeres. Recombinant human GH (r-hGH) was added to IVM medium and cumulus expansion, nuclear maturation, cytoplasmic maturation and embryo development were analyzed. Results: Cumulus expansion was highest in the presence of 1 and 10 ng/ml r-hGH. The addition of r-hGH during IVM increased the percentage of embryos progressing to at least the 9-16 cell stage. In a separate study, 100 ng/ml r-hGH was supplemented to IVM and embryo culture medium and no effect was observed. Conclusions: The presence of GH receptors along with increased cumulus expansion and embryos progressing to the 9-16 cell stage supports the hypothesis that r-hGH may be involved in oocyte maturation.
KW - Cumulus cells
KW - Growth hormone
KW - Non-human primate
UR - http://www.scopus.com/inward/record.url?scp=42549091671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42549091671&partnerID=8YFLogxK
U2 - 10.1007/s10815-008-9208-3
DO - 10.1007/s10815-008-9208-3
M3 - Article
C2 - 18278582
AN - SCOPUS:42549091671
VL - 25
SP - 145
EP - 158
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
SN - 1058-0468
IS - 4
ER -