Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Jenna K. Nyholt De Prada, Catherine A. VandeVoort

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose: The objective of this study was to use a nonhuman primate model to examine the effects of growth hormone (GH) on oocyte in vitro maturation (IVM). Methods: Immunocytochemistry confirmed the presence of GH receptors in rhesus cumulus oocyte complexes and the cytoplasm of embryonic blastomeres. Recombinant human GH (r-hGH) was added to IVM medium and cumulus expansion, nuclear maturation, cytoplasmic maturation and embryo development were analyzed. Results: Cumulus expansion was highest in the presence of 1 and 10 ng/ml r-hGH. The addition of r-hGH during IVM increased the percentage of embryos progressing to at least the 9-16 cell stage. In a separate study, 100 ng/ml r-hGH was supplemented to IVM and embryo culture medium and no effect was observed. Conclusions: The presence of GH receptors along with increased cumulus expansion and embryos progressing to the 9-16 cell stage supports the hypothesis that r-hGH may be involved in oocyte maturation.

Original languageEnglish (US)
Pages (from-to)145-158
Number of pages14
JournalJournal of Assisted Reproduction and Genetics
Volume25
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Macaca mulatta
Growth Hormone
Oocytes
Embryonic Development
Somatotropin Receptors
Embryonic Structures
In Vitro Oocyte Maturation Techniques
Blastomeres
Human Growth Hormone
Primates
Culture Media
Cytoplasm
Immunohistochemistry
In Vitro Techniques

Keywords

  • Cumulus cells
  • Growth hormone
  • Non-human primate

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics
  • Reproductive Medicine

Cite this

Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development. / Nyholt De Prada, Jenna K.; VandeVoort, Catherine A.

In: Journal of Assisted Reproduction and Genetics, Vol. 25, No. 4, 04.2008, p. 145-158.

Research output: Contribution to journalArticle

Nyholt De Prada, Jenna K. ; VandeVoort, Catherine A. / Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development. In: Journal of Assisted Reproduction and Genetics. 2008 ; Vol. 25, No. 4. pp. 145-158.
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