Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Jenna K. Nyholt De Prada, Catherine A. VandeVoort

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Purpose: The objective of this study was to use a nonhuman primate model to examine the effects of growth hormone (GH) on oocyte in vitro maturation (IVM). Methods: Immunocytochemistry confirmed the presence of GH receptors in rhesus cumulus oocyte complexes and the cytoplasm of embryonic blastomeres. Recombinant human GH (r-hGH) was added to IVM medium and cumulus expansion, nuclear maturation, cytoplasmic maturation and embryo development were analyzed. Results: Cumulus expansion was highest in the presence of 1 and 10 ng/ml r-hGH. The addition of r-hGH during IVM increased the percentage of embryos progressing to at least the 9-16 cell stage. In a separate study, 100 ng/ml r-hGH was supplemented to IVM and embryo culture medium and no effect was observed. Conclusions: The presence of GH receptors along with increased cumulus expansion and embryos progressing to the 9-16 cell stage supports the hypothesis that r-hGH may be involved in oocyte maturation.

Original languageEnglish (US)
Pages (from-to)145-158
Number of pages14
JournalJournal of Assisted Reproduction and Genetics
Issue number4
StatePublished - Apr 2008


  • Cumulus cells
  • Growth hormone
  • Non-human primate

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics
  • Reproductive Medicine


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