Growth hormone and gene expression of in vitro-matured rhesus macaque oocytes

Jenna K. Nyholt De Prada, Lori D. Kellam, Bela G. Patel, Keith E. Latham, Catherine A. Vandevoort

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Growth hormone (GH) in rhesus macaque in vitro oocyte maturation (IVM) has been shown to increase cumulus expansion and development of embryos to the 9-16 cell stage in response to 100 ng/ml recombinant human GH (r-hGH) supplementation during IVM. Although developmental endpoints for metaphase II (MII) oocytes and embryos are limited in the macaque, gene expression analysis can provide a mechanism to explore GH action on IVM. In addition, gene expression analysis may allow molecular events associated with improved cytoplasmic maturation to be detected. In this study, gene expression of specific mRNAs in MII oocytes and cumulus cells that have or have not been exposed to r-hGH during IVM was compared. In addition, mRNA expression was compared between in vitro and in vivo -matured metaphase II (MII) oocytes and germinal vesicle (GV)-stage oocytes. Only 2 of 17 genes, insulin-like growth factor 2 (IGF2) and steroidogenic acute regulator (STAR), showed increased mRNA expression in MII oocytes from the 100 ng/ml r-hGH treatment group compared with other IVM treatment groups, implicating insulin-like growth factor (IGF) and steroidogenesis pathways in the oocyte response to GH. The importance of IGF2 is notable, as expression of IGF1 was not detected in macaque GV-stage or MII oocytes or cumulus cells.

Original languageEnglish (US)
Pages (from-to)353-362
Number of pages10
JournalMolecular Reproduction and Development
Volume77
Issue number4
DOIs
StatePublished - Apr 2010

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Macaca mulatta
In Vitro Oocyte Maturation Techniques
Growth Hormone
Oocytes
Metaphase
Gene Expression
Somatomedins
Cumulus Cells
Macaca
Messenger RNA
Human Growth Hormone
In Vitro Techniques
Embryonic Development
Embryonic Structures
Therapeutics
Genes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Nyholt De Prada, J. K., Kellam, L. D., Patel, B. G., Latham, K. E., & Vandevoort, C. A. (2010). Growth hormone and gene expression of in vitro-matured rhesus macaque oocytes. Molecular Reproduction and Development, 77(4), 353-362. https://doi.org/10.1002/mrd.21152

Growth hormone and gene expression of in vitro-matured rhesus macaque oocytes. / Nyholt De Prada, Jenna K.; Kellam, Lori D.; Patel, Bela G.; Latham, Keith E.; Vandevoort, Catherine A.

In: Molecular Reproduction and Development, Vol. 77, No. 4, 04.2010, p. 353-362.

Research output: Contribution to journalArticle

Nyholt De Prada, JK, Kellam, LD, Patel, BG, Latham, KE & Vandevoort, CA 2010, 'Growth hormone and gene expression of in vitro-matured rhesus macaque oocytes', Molecular Reproduction and Development, vol. 77, no. 4, pp. 353-362. https://doi.org/10.1002/mrd.21152
Nyholt De Prada, Jenna K. ; Kellam, Lori D. ; Patel, Bela G. ; Latham, Keith E. ; Vandevoort, Catherine A. / Growth hormone and gene expression of in vitro-matured rhesus macaque oocytes. In: Molecular Reproduction and Development. 2010 ; Vol. 77, No. 4. pp. 353-362.
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