Growth factors for fetal and adult human astrocytes in culture

Wee Yong Voon Wee Yong, Seung U. Kim, David E Pleasure

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Using a recently developed method for the determination of cell proliferation in culture, we assessed the effects of several growth factors on their capability to promote proliferation of astrocytes derived from human fetuses or adults. Factors tested included fibroblast growth factor, epidermal growth factor, platelet-derived growth factor, glial growth factor from bovine pituitary, nerve growth factor, interleukin-2, dibutyryl cyclic adenosine monophosphate, and 4β-phorbol 12,13-dibytyrate. The results show that astrocytes from human adults do not undergo proliferation in vitro even in the presence of these agents and that in the case of fetal astrocytes, only glial growth factor from bovine pituitary, platelet-derived growth factor and the phorbol ester were mitogenic. In addition, the capability of fetal astrocytes to proliferate appears to decrease with the increasing length of time they have been maintained in culture.

Original languageEnglish (US)
Pages (from-to)59-66
Number of pages8
JournalBrain Research
Volume444
Issue number1
DOIs
StatePublished - Mar 15 1988
Externally publishedYes

Keywords

  • Astrocyte
  • Bromodeoxyuridine
  • Glial growth factor from bovine pituitary
  • Human
  • Mitogen
  • Phorbol ester
  • Platelet-derived growth factor
  • Tissue culture

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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