Growth factor impact on articular cartilage subpopulations

Eric M. Darling, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We have examined the effects of growth factor stimulation on superficial and growth zone chondrocyte populations. Zonal articular chondrocytes from 8-month-old Spanish goat distal femurs were plated in monolayer cultures and stimulated by using insulin-like growth factor I (IGF-I), basic fibroblast growth factor (bFGF), and transforming growth factor-β1 (TGF-β1). Gene expression for collagen I and II, aggrecan, and superficial zone protein were evaluated every week for 3 weeks. Finally, proteoglycan and collagen deposition were measured for each experimental group. Major differences existed in the behavior of superficial and growth zone chondrocytes, the most apparent being the higher capacity for protein synthesis by the growth zone population. Variations also existed regarding growth factor treatment. TGF-β1 had the greatest effect on proliferation over 8 days. With respect to differentiation, IGF-I increased average collagen II gene expression in the growth zone populations in comparison with growth zone controls. IGF-I increased aggrecan gene expression for the same groups. Superficial zone populations exhibited lower collagen II, collagen I, and aggrecan gene expression than the growth zone populations under all conditions. However, superficial zone protein expression was dramatically elevated in superficial zone populations by TGF-β1. Collagen I expression showed a general increase under all conditions compared with initial values. Combined biosynthesis results showed that the superficial populations secreted little to no collagen, especially collagen II, in comparison with their growth zone counterparts. Glycosaminoglycan production was also much lower than for the growth zone groups. TGF-β1 and IGF-I increased collagen II production in the growth zone populations. TGF-β1 increased glycosaminoglycan secretions in the superficial zone populations and in the growth zone populations, whereas IGF-I produced an increase in glycosaminoglycan secretion only in the growth zone populations. Thus, growth factors elicit different proliferation, gene expression, and biosynthesis responses from zonal chondrocyte subpopulations.

Original languageEnglish (US)
Pages (from-to)463-473
Number of pages11
JournalCell and Tissue Research
Volume322
Issue number3
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Cartilage
Articular Cartilage
Population Growth
Intercellular Signaling Peptides and Proteins
Collagen
Transforming Growth Factors
Insulin-Like Growth Factor I
Chondrocytes
Gene expression
Aggrecans
Gene Expression
Glycosaminoglycans
Growth
Population
Biosynthesis
Proteins
Fibroblast Growth Factor 2
Proteoglycans
Goats
Femur

Keywords

  • Chondrocyte
  • Differentiation
  • FGF
  • Goat
  • Growth
  • IGF
  • TGF
  • Zones

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Growth factor impact on articular cartilage subpopulations. / Darling, Eric M.; Athanasiou, Kyriacos A.

In: Cell and Tissue Research, Vol. 322, No. 3, 12.2005, p. 463-473.

Research output: Contribution to journalArticle

Darling, Eric M. ; Athanasiou, Kyriacos A. / Growth factor impact on articular cartilage subpopulations. In: Cell and Tissue Research. 2005 ; Vol. 322, No. 3. pp. 463-473.
@article{ad75796e30754601acbbd58b7b3aae3c,
title = "Growth factor impact on articular cartilage subpopulations",
abstract = "We have examined the effects of growth factor stimulation on superficial and growth zone chondrocyte populations. Zonal articular chondrocytes from 8-month-old Spanish goat distal femurs were plated in monolayer cultures and stimulated by using insulin-like growth factor I (IGF-I), basic fibroblast growth factor (bFGF), and transforming growth factor-β1 (TGF-β1). Gene expression for collagen I and II, aggrecan, and superficial zone protein were evaluated every week for 3 weeks. Finally, proteoglycan and collagen deposition were measured for each experimental group. Major differences existed in the behavior of superficial and growth zone chondrocytes, the most apparent being the higher capacity for protein synthesis by the growth zone population. Variations also existed regarding growth factor treatment. TGF-β1 had the greatest effect on proliferation over 8 days. With respect to differentiation, IGF-I increased average collagen II gene expression in the growth zone populations in comparison with growth zone controls. IGF-I increased aggrecan gene expression for the same groups. Superficial zone populations exhibited lower collagen II, collagen I, and aggrecan gene expression than the growth zone populations under all conditions. However, superficial zone protein expression was dramatically elevated in superficial zone populations by TGF-β1. Collagen I expression showed a general increase under all conditions compared with initial values. Combined biosynthesis results showed that the superficial populations secreted little to no collagen, especially collagen II, in comparison with their growth zone counterparts. Glycosaminoglycan production was also much lower than for the growth zone groups. TGF-β1 and IGF-I increased collagen II production in the growth zone populations. TGF-β1 increased glycosaminoglycan secretions in the superficial zone populations and in the growth zone populations, whereas IGF-I produced an increase in glycosaminoglycan secretion only in the growth zone populations. Thus, growth factors elicit different proliferation, gene expression, and biosynthesis responses from zonal chondrocyte subpopulations.",
keywords = "Chondrocyte, Differentiation, FGF, Goat, Growth, IGF, TGF, Zones",
author = "Darling, {Eric M.} and Athanasiou, {Kyriacos A.}",
year = "2005",
month = "12",
doi = "10.1007/s00441-005-0020-4",
language = "English (US)",
volume = "322",
pages = "463--473",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Growth factor impact on articular cartilage subpopulations

AU - Darling, Eric M.

AU - Athanasiou, Kyriacos A.

PY - 2005/12

Y1 - 2005/12

N2 - We have examined the effects of growth factor stimulation on superficial and growth zone chondrocyte populations. Zonal articular chondrocytes from 8-month-old Spanish goat distal femurs were plated in monolayer cultures and stimulated by using insulin-like growth factor I (IGF-I), basic fibroblast growth factor (bFGF), and transforming growth factor-β1 (TGF-β1). Gene expression for collagen I and II, aggrecan, and superficial zone protein were evaluated every week for 3 weeks. Finally, proteoglycan and collagen deposition were measured for each experimental group. Major differences existed in the behavior of superficial and growth zone chondrocytes, the most apparent being the higher capacity for protein synthesis by the growth zone population. Variations also existed regarding growth factor treatment. TGF-β1 had the greatest effect on proliferation over 8 days. With respect to differentiation, IGF-I increased average collagen II gene expression in the growth zone populations in comparison with growth zone controls. IGF-I increased aggrecan gene expression for the same groups. Superficial zone populations exhibited lower collagen II, collagen I, and aggrecan gene expression than the growth zone populations under all conditions. However, superficial zone protein expression was dramatically elevated in superficial zone populations by TGF-β1. Collagen I expression showed a general increase under all conditions compared with initial values. Combined biosynthesis results showed that the superficial populations secreted little to no collagen, especially collagen II, in comparison with their growth zone counterparts. Glycosaminoglycan production was also much lower than for the growth zone groups. TGF-β1 and IGF-I increased collagen II production in the growth zone populations. TGF-β1 increased glycosaminoglycan secretions in the superficial zone populations and in the growth zone populations, whereas IGF-I produced an increase in glycosaminoglycan secretion only in the growth zone populations. Thus, growth factors elicit different proliferation, gene expression, and biosynthesis responses from zonal chondrocyte subpopulations.

AB - We have examined the effects of growth factor stimulation on superficial and growth zone chondrocyte populations. Zonal articular chondrocytes from 8-month-old Spanish goat distal femurs were plated in monolayer cultures and stimulated by using insulin-like growth factor I (IGF-I), basic fibroblast growth factor (bFGF), and transforming growth factor-β1 (TGF-β1). Gene expression for collagen I and II, aggrecan, and superficial zone protein were evaluated every week for 3 weeks. Finally, proteoglycan and collagen deposition were measured for each experimental group. Major differences existed in the behavior of superficial and growth zone chondrocytes, the most apparent being the higher capacity for protein synthesis by the growth zone population. Variations also existed regarding growth factor treatment. TGF-β1 had the greatest effect on proliferation over 8 days. With respect to differentiation, IGF-I increased average collagen II gene expression in the growth zone populations in comparison with growth zone controls. IGF-I increased aggrecan gene expression for the same groups. Superficial zone populations exhibited lower collagen II, collagen I, and aggrecan gene expression than the growth zone populations under all conditions. However, superficial zone protein expression was dramatically elevated in superficial zone populations by TGF-β1. Collagen I expression showed a general increase under all conditions compared with initial values. Combined biosynthesis results showed that the superficial populations secreted little to no collagen, especially collagen II, in comparison with their growth zone counterparts. Glycosaminoglycan production was also much lower than for the growth zone groups. TGF-β1 and IGF-I increased collagen II production in the growth zone populations. TGF-β1 increased glycosaminoglycan secretions in the superficial zone populations and in the growth zone populations, whereas IGF-I produced an increase in glycosaminoglycan secretion only in the growth zone populations. Thus, growth factors elicit different proliferation, gene expression, and biosynthesis responses from zonal chondrocyte subpopulations.

KW - Chondrocyte

KW - Differentiation

KW - FGF

KW - Goat

KW - Growth

KW - IGF

KW - TGF

KW - Zones

UR - http://www.scopus.com/inward/record.url?scp=27944506373&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27944506373&partnerID=8YFLogxK

U2 - 10.1007/s00441-005-0020-4

DO - 10.1007/s00441-005-0020-4

M3 - Article

C2 - 16047167

AN - SCOPUS:27944506373

VL - 322

SP - 463

EP - 473

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 3

ER -