Growth factor effects on costal chondrocytes for tissue engineering fibrocartilage

D. E. Johns, K. A. Athanasiou

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Tissue-engineered fibrocartilage could become a feasible option for replacing tissues such as the knee meniscus or temporomandibular joint disc. This study employed five growth factors (insulin-like growth factor-I, transforming growth factor-β1, epidermal growth factor, platelet-derived growth factor-BB, and basic fibroblast growth factor) in a scaffoldless approach with costal chondrocytes, attempting to improve biochemical and mechanical properties of engineered constructs. Samples were quantitatively assessed for total collagen, glycosaminoglycans, collagen type I, collagen type II, cells, compressive properties, and tensile properties at two time points. Most treated constructs had lower biomechanical and biochemical properties than the controls with no growth factors, suggesting a detrimental effect, but the treatment with insulin-like growth factor-I tended to improve the constructs. Additionally, the 6-week time point was consistently better than that at 3 weeks, with total collagen, glycosaminoglycans, and aggregate modulus doubling during this time. Further optimization of the time in culture and exogenous stimuli will be important in making a more functional replacement tissue.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalCell and Tissue Research
Issue number3
StatePublished - Sep 2008
Externally publishedYes


  • Fibrocartilage
  • Goat
  • Growth factors
  • Knee meniscus
  • Temporomandibular joint disc
  • Tissue engineering

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology


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