Growth factor effects on costal chondrocytes for tissue engineering fibrocartilage

D. E. Johns, K. A. Athanasiou

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Tissue-engineered fibrocartilage could become a feasible option for replacing tissues such as the knee meniscus or temporomandibular joint disc. This study employed five growth factors (insulin-like growth factor-I, transforming growth factor-β1, epidermal growth factor, platelet-derived growth factor-BB, and basic fibroblast growth factor) in a scaffoldless approach with costal chondrocytes, attempting to improve biochemical and mechanical properties of engineered constructs. Samples were quantitatively assessed for total collagen, glycosaminoglycans, collagen type I, collagen type II, cells, compressive properties, and tensile properties at two time points. Most treated constructs had lower biomechanical and biochemical properties than the controls with no growth factors, suggesting a detrimental effect, but the treatment with insulin-like growth factor-I tended to improve the constructs. Additionally, the 6-week time point was consistently better than that at 3 weeks, with total collagen, glycosaminoglycans, and aggregate modulus doubling during this time. Further optimization of the time in culture and exogenous stimuli will be important in making a more functional replacement tissue.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalCell and Tissue Research
Volume333
Issue number3
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Fibrocartilage
Tissue Engineering
Chondrocytes
Tissue engineering
Intercellular Signaling Peptides and Proteins
Tissue
Glycosaminoglycans
Insulin-Like Growth Factor I
Collagen
Collagen Type II
Temporomandibular Joint Disc
Transforming Growth Factors
Fibroblast Growth Factor 2
Collagen Type I
Tensile properties
Epidermal Growth Factor
Knee Joint
Mechanical properties

Keywords

  • Fibrocartilage
  • Goat
  • Growth factors
  • Knee meniscus
  • Temporomandibular joint disc
  • Tissue engineering

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Growth factor effects on costal chondrocytes for tissue engineering fibrocartilage. / Johns, D. E.; Athanasiou, K. A.

In: Cell and Tissue Research, Vol. 333, No. 3, 09.2008, p. 439-447.

Research output: Contribution to journalArticle

Johns, D. E. ; Athanasiou, K. A. / Growth factor effects on costal chondrocytes for tissue engineering fibrocartilage. In: Cell and Tissue Research. 2008 ; Vol. 333, No. 3. pp. 439-447.
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