Growth, differentiation, and biochemical signatures of rhesus monkey mesenchymal stem cells

Brandon S. Kim, Charles C Lee, Jared E. Christensen, Thomas R Huser, James W Chan, Alice F Tarantal

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The goal of this study was to compare the growth and differentiation potential of rhesus monkey mesenchymal stem cells (rhMSCs) from different age groups (fetal, newborn, infant, juvenile), and to use confocal micro-Raman spectroscopy to assess the intrinsic biomolecular profiles of individual rhMSCs. Results indicated that fetal cells had significantly shorter population doubling times during the log growth phase (23.3 ± 1.3 h) and greater population doubling times (66.5 ± 6.5) when compared to other age groups (newborn 51.9 ± 2.3, infant 38.2 ± 3.1, juvenile 40.7 ± 4.1). Fetal rhMSCs also differentiated toward osteogenic and adipogenic lineages at a faster rate when compared to cells from older animals. The Raman spectral analysis showed greater DNA and lower protein concentration in fetal compared to juvenile rhMSCs, although the spectra from different age groups shared many similar features. Additionally, principal component analysis (PCA), which is used to discriminate between rhMSCs, supported prior findings that suggested that cultured rhMSCs consist of a heterogeneous cell population. Although the growth potential of rhMSCs from the younger age groups was confirmed, further studies will be necessary to fully explore the potential usefulness of Raman micro-spectroscopy to characterize stem and progenitor cells such as rhMSCs.

Original languageEnglish (US)
Pages (from-to)185-198
Number of pages14
JournalStem Cells and Development
Volume17
Issue number1
DOIs
StatePublished - Feb 1 2008

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Macaca mulatta
Mesenchymal Stromal Cells
Growth
Age Groups
Raman Spectrum Analysis
Stem Cells
Newborn Infant
Population
Principal Component Analysis
DNA
Proteins

ASJC Scopus subject areas

  • Hematology

Cite this

Growth, differentiation, and biochemical signatures of rhesus monkey mesenchymal stem cells. / Kim, Brandon S.; Lee, Charles C; Christensen, Jared E.; Huser, Thomas R; Chan, James W; Tarantal, Alice F.

In: Stem Cells and Development, Vol. 17, No. 1, 01.02.2008, p. 185-198.

Research output: Contribution to journalArticle

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