Abstract
Binding isotope effects for l-aspartate reacting with the inactive K258A mutant of PLP-dependent aspartate aminotransferase to give a stable external aldimine intermediate are reported. They provide direct evidence for electronic ground-state destabilization via hyperconjugation. The smaller equilibrium isotope effect with deazaPLP-reconstituted K258A indicates that the pyridine nitrogen plays an important role in labilizing the Cα-H bond.
Original language | English (US) |
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Pages (from-to) | 8436-8438 |
Number of pages | 3 |
Journal | Journal of the American Chemical Society |
Volume | 134 |
Issue number | 20 |
DOIs | |
State | Published - May 23 2012 |
ASJC Scopus subject areas
- Chemistry(all)
- Catalysis
- Biochemistry
- Colloid and Surface Chemistry