TY - JOUR
T1 - Gross morphology, histology, and ultrastructure of the olfactory rosette of a critically endangered indicator species, the Delta Smelt, Hypomesus transpacificus
AU - Triana-Garcia, Pedro Alejandro
AU - Nevitt, Gabrielle A.
AU - Pesavento, Joseph B.
AU - Teh, Swee J.
N1 - Funding Information:
We are grateful with Dr. Stuart Meyers for kindly allowing us to access the microscope used to acquire most of the pictures for this work. Likewise, we thank Dr. Tien-Chieh Hung and Luke Ellison from FCCL for providing the fish for this study. We also thank the anonymous reviewers who helped to improve this manuscript with their insightful comments. This work was supported by the Delta Stewardship Council Delta Science Program (R/SF-92, Grant number 5298) and by the US Bureau of Reclamation R17AC00129. GAN was supported by CA-D-NPB-3509-H. The contents of this research do not necessarily reflect the views and policies of the Delta Stewardship Council and US Bureau of Reclamation, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021
Y1 - 2021
N2 - The Delta Smelt (Hypomesus transpacificus) is a small, semi-anadromous fish native to the San Francisco Bay-Delta Estuary and has been declared as critically endangered. Their olfactory biology, in particular, is poorly understood and a basic description of their sensory anatomy is needed to advance our understanding of the sensory ecology of species to inform conservation efforts to manage and protect them. We provide a description of the gross morphology, histological, immunohistochemical, and ultrastructural features of the olfactory rosette in this fish and discuss some of the functional implications in relation to olfactory ability. We show that Delta Smelt have a multilamellar olfactory rosette with allometric growth. Calretinin immunohistochemistry revealed a diffuse distribution of olfactory receptor neurons within the epithelium. Ciliated, microvillous and crypt neurons were clearly identified using morphological and immunohistochemical features. The olfactory neurons were supported by robust ciliated and secretory sustentacular cells. Although the sense of smell has been overlooked in Delta Smelt, we conclude that the olfactory epithelium has many characteristics of macrosmatic fish. With this study, we provide a foundation for future research into the sensory ecology of this imperiled fish.
AB - The Delta Smelt (Hypomesus transpacificus) is a small, semi-anadromous fish native to the San Francisco Bay-Delta Estuary and has been declared as critically endangered. Their olfactory biology, in particular, is poorly understood and a basic description of their sensory anatomy is needed to advance our understanding of the sensory ecology of species to inform conservation efforts to manage and protect them. We provide a description of the gross morphology, histological, immunohistochemical, and ultrastructural features of the olfactory rosette in this fish and discuss some of the functional implications in relation to olfactory ability. We show that Delta Smelt have a multilamellar olfactory rosette with allometric growth. Calretinin immunohistochemistry revealed a diffuse distribution of olfactory receptor neurons within the epithelium. Ciliated, microvillous and crypt neurons were clearly identified using morphological and immunohistochemical features. The olfactory neurons were supported by robust ciliated and secretory sustentacular cells. Although the sense of smell has been overlooked in Delta Smelt, we conclude that the olfactory epithelium has many characteristics of macrosmatic fish. With this study, we provide a foundation for future research into the sensory ecology of this imperiled fish.
KW - Fish
KW - Immunohistochemistry
KW - Neuroanatomy
KW - Olfaction
KW - Sensory ecology
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U2 - 10.1007/s00359-021-01500-7
DO - 10.1007/s00359-021-01500-7
M3 - Article
C2 - 34156533
AN - SCOPUS:85108656447
JO - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology
JF - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology
SN - 0340-7594
ER -