Granulocyte colony-stimulating factor mobilizes more dendritic cell subsets than granulocyte-macrophage colony-stimulating factor with no polarization of dendritic cell subsets in normal donors

Paul J. Shaughnessy, Carlos Bachier, Charles F. LeMaistre, Cagla Akay, Bradley H Pollock, Yair Gazitt

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Dendritic cells (DCs) are effective antigen-presenting cells. We hypothesized that increasing the DC populations in donor lymphocyte infusions (DLIs) may augment the graft versus malignancy effect, particularly if granulocyte-macrophage colony-stimulating factor (GM-CSF) mobilization resulted in increased precursor dendritic cell (pDC) 1 cells. Mature DCs, pDC1 cells, pDC2 cells, and CD34+ cells from the same donor were compared after granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood stem cell collections and GM-CSF mobilized DLI collections. Mobilization with G-CSF resulted in up to a 10-fold larger number of CD34+ cells per kg and a 3-5-fold larger number of mature DCs, pDC1 cells, and pDC2 cells within the same donor compared with GM-CSF. The ratio of pDC1 to pDC2 in each donor remained constant with either cytokine. In this small sample of normal donors, it appears that G-CSF mobilizes more CD34+ cells, mature DCs, pDC1 cells, and pDC2 cells within the same donor than does GM-CSF, with no significant polarization by G-CSF or GM-CSF for either pDC1 or pDC2 cells.

Original languageEnglish (US)
Pages (from-to)1789-1797
Number of pages9
JournalStem Cells
Volume24
Issue number7
DOIs
StatePublished - Jul 2006
Externally publishedYes

Keywords

  • Dentritic cells
  • Graft versus host disease
  • Granulocyte colony-stimulating factor
  • Granulocyte-macrophage colony-stimulating factor
  • Mobilization

ASJC Scopus subject areas

  • Cell Biology

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