Abstract
We investigated the exchange of water and macromolecules across venular microvessels after permeability was increased. Quantitative fluorescence microscopy was used to measure albumin permeability coefficients in individually perfused microvessels of decerebrate frogs. Control permeability coefficient was 2.3 ± 0.25 x 10--7 cm/s. Solvent drag increased the apparent solute permeability coefficient (Ps)) by 0.57 ± 0.05 x 10-7 cm/s for each cmH2O increase in microvessel pressure. The divalent cation ionophore A23187 (0.1-5 μM) produced a transient increase in P(s) to a peak value (within 1-3 min), followed (after 4-8 min) by a sustained increase in permeability (16-43% of peak values). Peak values of P(s) were 13 and 80 times control for 0.1 and 5 μM A23187, respectively. Both diffusion and solvent drag contributed to the sustained increase in P(s). The equivalent pore radius of the structures determining diffusion and solvent drag was ≤25 nm during the sustained increase in permeability, smaller than observed gaps between adjacent endothelial cells. The basement membrane and a fibrous matrix secreted by endothelial cells into the gaps may offer resistance to exchange in the high permeability state.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Heart and Circulatory Physiology |
| Volume | 258 |
| Issue number | 2 27-2 |
| State | Published - 1990 |
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Keywords
- calcium modulation of capillary permeability
- fiber matrix
- inflammatory mediators
- pore models
- single capillaries
ASJC Scopus subject areas
- Physiology
Cite this
Graded modulation of frog microvessel permeability to albumin using ionophore A23187. / Curry, F. E.; Joyner, W. L.; Rutledge, John C.
In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 258, No. 2 27-2, 1990.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Graded modulation of frog microvessel permeability to albumin using ionophore A23187
AU - Curry, F. E.
AU - Joyner, W. L.
AU - Rutledge, John C
PY - 1990
Y1 - 1990
N2 - We investigated the exchange of water and macromolecules across venular microvessels after permeability was increased. Quantitative fluorescence microscopy was used to measure albumin permeability coefficients in individually perfused microvessels of decerebrate frogs. Control permeability coefficient was 2.3 ± 0.25 x 10--7 cm/s. Solvent drag increased the apparent solute permeability coefficient (Ps)) by 0.57 ± 0.05 x 10-7 cm/s for each cmH2O increase in microvessel pressure. The divalent cation ionophore A23187 (0.1-5 μM) produced a transient increase in P(s) to a peak value (within 1-3 min), followed (after 4-8 min) by a sustained increase in permeability (16-43% of peak values). Peak values of P(s) were 13 and 80 times control for 0.1 and 5 μM A23187, respectively. Both diffusion and solvent drag contributed to the sustained increase in P(s). The equivalent pore radius of the structures determining diffusion and solvent drag was ≤25 nm during the sustained increase in permeability, smaller than observed gaps between adjacent endothelial cells. The basement membrane and a fibrous matrix secreted by endothelial cells into the gaps may offer resistance to exchange in the high permeability state.
AB - We investigated the exchange of water and macromolecules across venular microvessels after permeability was increased. Quantitative fluorescence microscopy was used to measure albumin permeability coefficients in individually perfused microvessels of decerebrate frogs. Control permeability coefficient was 2.3 ± 0.25 x 10--7 cm/s. Solvent drag increased the apparent solute permeability coefficient (Ps)) by 0.57 ± 0.05 x 10-7 cm/s for each cmH2O increase in microvessel pressure. The divalent cation ionophore A23187 (0.1-5 μM) produced a transient increase in P(s) to a peak value (within 1-3 min), followed (after 4-8 min) by a sustained increase in permeability (16-43% of peak values). Peak values of P(s) were 13 and 80 times control for 0.1 and 5 μM A23187, respectively. Both diffusion and solvent drag contributed to the sustained increase in P(s). The equivalent pore radius of the structures determining diffusion and solvent drag was ≤25 nm during the sustained increase in permeability, smaller than observed gaps between adjacent endothelial cells. The basement membrane and a fibrous matrix secreted by endothelial cells into the gaps may offer resistance to exchange in the high permeability state.
KW - calcium modulation of capillary permeability
KW - fiber matrix
KW - inflammatory mediators
KW - pore models
KW - single capillaries
UR - http://www.scopus.com/inward/record.url?scp=0025337886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025337886&partnerID=8YFLogxK
M3 - Article
C2 - 2106799
AN - SCOPUS:0025337886
VL - 258
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 2 27-2
ER -