Graded modulation of frog microvessel permeability to albumin using ionophore A23187

F. E. Curry, W. L. Joyner, John C Rutledge

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We investigated the exchange of water and macromolecules across venular microvessels after permeability was increased. Quantitative fluorescence microscopy was used to measure albumin permeability coefficients in individually perfused microvessels of decerebrate frogs. Control permeability coefficient was 2.3 ± 0.25 x 10--7 cm/s. Solvent drag increased the apparent solute permeability coefficient (Ps)) by 0.57 ± 0.05 x 10-7 cm/s for each cmH2O increase in microvessel pressure. The divalent cation ionophore A23187 (0.1-5 μM) produced a transient increase in P(s) to a peak value (within 1-3 min), followed (after 4-8 min) by a sustained increase in permeability (16-43% of peak values). Peak values of P(s) were 13 and 80 times control for 0.1 and 5 μM A23187, respectively. Both diffusion and solvent drag contributed to the sustained increase in P(s). The equivalent pore radius of the structures determining diffusion and solvent drag was ≤25 nm during the sustained increase in permeability, smaller than observed gaps between adjacent endothelial cells. The basement membrane and a fibrous matrix secreted by endothelial cells into the gaps may offer resistance to exchange in the high permeability state.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume258
Issue number2 27-2
StatePublished - 1990

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Ionophores
Calcimycin
Microvessels
Anura
Albumins
Permeability
Endothelial Cells
Divalent Cations
Fluorescence Microscopy
Basement Membrane
Pressure
Water

Keywords

  • calcium modulation of capillary permeability
  • fiber matrix
  • inflammatory mediators
  • pore models
  • single capillaries

ASJC Scopus subject areas

  • Physiology

Cite this

Graded modulation of frog microvessel permeability to albumin using ionophore A23187. / Curry, F. E.; Joyner, W. L.; Rutledge, John C.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 258, No. 2 27-2, 1990.

Research output: Contribution to journalArticle

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