Abstract
Glycosyltransferases (GTs) are powerful tools for the synthesis of complex and biologically-important carbohydrates. Wild-type GTs may not have all the properties and functions that are desired for largescale production of carbohydrates that exist in nature and those with non-natural modifications. With the increasing availability of crystal structures of GTs, especially those in the presence of donor and acceptor analogues, crystal structure-guided rational design has been quite successful in obtaining mutants with desired functionalities. With current limited understanding of the structure-activity relationship of GTs, directed evolution continues to be a useful approach for generating additional mutants with functionality that can be screened for in a high-throughput format. Mutating the amino acid residues constituting or close to the substrate-binding sites of GTs by structure-guided directed evolution (SGDE) further explores the biotechnological potential of GTs that can only be realized through enzyme engineering. This mini-review discusses the progress made towards GT engineering and the lessons learned for future engineering efforts and assay development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 129-142 |
| Number of pages | 14 |
| Journal | Biochemical Society Transactions |
| Volume | 44 |
| DOIs | |
| State | Published - Feb 15 2016 |
Fingerprint
Keywords
- Carbohydrate
- Directed evolution
- Enzymatic synthesis
- Glycosyltransferase
- Mutagenesis
- Protein engineering
ASJC Scopus subject areas
- Biochemistry
Cite this
Glycosyltransferase engineering for carbohydrate synthesis. / McArthur, John B.; Chen, Xi.
In: Biochemical Society Transactions, Vol. 44, 15.02.2016, p. 129-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycosyltransferase engineering for carbohydrate synthesis
AU - McArthur, John B.
AU - Chen, Xi
PY - 2016/2/15
Y1 - 2016/2/15
N2 - Glycosyltransferases (GTs) are powerful tools for the synthesis of complex and biologically-important carbohydrates. Wild-type GTs may not have all the properties and functions that are desired for largescale production of carbohydrates that exist in nature and those with non-natural modifications. With the increasing availability of crystal structures of GTs, especially those in the presence of donor and acceptor analogues, crystal structure-guided rational design has been quite successful in obtaining mutants with desired functionalities. With current limited understanding of the structure-activity relationship of GTs, directed evolution continues to be a useful approach for generating additional mutants with functionality that can be screened for in a high-throughput format. Mutating the amino acid residues constituting or close to the substrate-binding sites of GTs by structure-guided directed evolution (SGDE) further explores the biotechnological potential of GTs that can only be realized through enzyme engineering. This mini-review discusses the progress made towards GT engineering and the lessons learned for future engineering efforts and assay development.
AB - Glycosyltransferases (GTs) are powerful tools for the synthesis of complex and biologically-important carbohydrates. Wild-type GTs may not have all the properties and functions that are desired for largescale production of carbohydrates that exist in nature and those with non-natural modifications. With the increasing availability of crystal structures of GTs, especially those in the presence of donor and acceptor analogues, crystal structure-guided rational design has been quite successful in obtaining mutants with desired functionalities. With current limited understanding of the structure-activity relationship of GTs, directed evolution continues to be a useful approach for generating additional mutants with functionality that can be screened for in a high-throughput format. Mutating the amino acid residues constituting or close to the substrate-binding sites of GTs by structure-guided directed evolution (SGDE) further explores the biotechnological potential of GTs that can only be realized through enzyme engineering. This mini-review discusses the progress made towards GT engineering and the lessons learned for future engineering efforts and assay development.
KW - Carbohydrate
KW - Directed evolution
KW - Enzymatic synthesis
KW - Glycosyltransferase
KW - Mutagenesis
KW - Protein engineering
UR - http://www.scopus.com/inward/record.url?scp=84957885647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957885647&partnerID=8YFLogxK
U2 - 10.1042/BST20150200
DO - 10.1042/BST20150200
M3 - Article
VL - 44
SP - 129
EP - 142
JO - Biochemical Society Transactions
JF - Biochemical Society Transactions
SN - 0300-5127
ER -