Glycosphingolipid carriers of carbohydrate antigens of human myeloid cells recognized by monoclonal antibodies

Kei ichi Uemura, Bruce A. Macher, Michael DeGregorio, Peter Scudder, Joanne Buehler, Walter Knapp, Ten Feizi

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Six monoclonal antibodies with known specificities for the carbohydrate antigens i, X or Y, and seven anti-myeloid antibodies (determinants unknown) selected for their differing reaction patterns with human leucocytes were tested in chromatogram binding assays for reactions with myeloid cell glycolipids derived from normal human granulocytes and chronic myelogenous leukemia cells. Antigenicities were found exclusively on minor glycolipids which were barely or not at all detectable with orcinol-sulphuric acid stain. Among these, a neutral glycosphingolipid bound the anti-i antibody Den and chromatographed as the ceramide octasaccharide, Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glc-Cer. Several species of neutral glycosphingolipids with six to more than ten monosaccharides were detected which carry the X antigen and others the Y antigen: Galβ1 → 4(Fucα1 → 3)GlcNAc and Fucα1 → 2Galβ1 → 4(Fucα1 → 3)GlcNAc, respectively. In addition, three new types of carbohydrate specificities were detected among the myeloid cell glycolipids. Two were associated with neutral glycolipids: the first, recognised by anti-myeloid antibodies VIM-1 and VIM-10, was expressed on a distinct set of glycolipids with six or more monosaccharides, and the second, recognized by VIM-8, was expressed on glycolipids with more than ten monosaccharides. The third specificity, recognised by the anti-myeloid antibody VIM-2, was expressed on slow migrating sialoglycolipids with backbone structures of the poly-N-acetyllactosamine type that are susceptible to degradation with endo-β-galactosidase. Thus, we conclude that the i and Y antigens occur among the glycolipids of normal myeloid and chronic myelogenous leukemia cells and that a high proportion of hybridoma antibodies raised against differentiation antigens of myeloid cells are directed at carbohydrate structures.

Original languageEnglish (US)
Pages (from-to)26-36
Number of pages11
JournalBBA - Molecular Cell Research
Volume846
Issue number1
DOIs
StatePublished - Jul 30 1985
Externally publishedYes

Keywords

  • (Human myeloid cell)
  • Carbohydrate antigen
  • Glycosphingolipid
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

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    Uemura, K. I., Macher, B. A., DeGregorio, M., Scudder, P., Buehler, J., Knapp, W., & Feizi, T. (1985). Glycosphingolipid carriers of carbohydrate antigens of human myeloid cells recognized by monoclonal antibodies. BBA - Molecular Cell Research, 846(1), 26-36. https://doi.org/10.1016/0167-4889(85)90106-5