Glycoproteins of the vitelline envelope of amphibian oocyte

Biological and molecular characterization of ZPC component (gp41) in Bufo arenarum

Gustavo Barisone, Darío Krapf, Florencia Correa-Fiz, Silvia E. Arranz, Marcelo O. Cabada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The vitelline envelope (VE) participates in sperm-egg interactions during the first steps of fertilization. In Bufo arenarum, this envelope is composed of at least four glycoproteins, with molecular masses of 120, 75, 41, and 38 kDa and molar ratio of 1:1.3:7.4:4.8, respectively. These components were isolated and covalently coupled to silanized glass slides in order to study their sperm-binding capacity. When considering the molar ratio of the glycoproteins in the egg-envelope and assuming that each protein is monovalent for sperm, the assay showed that gp41 and gp38 possess 55 and 25% of total sperm-binding activity. We obtained a full-length cDNA of gp41 (ZPC), comprising a sequence for 486 amino acids, with 43.3% homology with Xenopus laevis ZPC. As in the case of mammalian ZP3 and Xenopus ZPC, Bufo ZPC presented a furin-like (convertase) and a C-terminal transmembrane domain (TMD) reflecting common biosynthetic and secretory pathways. As it was reported for some fishes, we obtained evidence that suggests the presence of more than one zpc gene in Bufo genome, based on different partial cDNA sequences of zpc, Southern blots and two-dimensional SDS-PAGE of deglycosylated egg-envelope components. As far as we are aware, this is the first observation of the presence of different zpc genes in an Amphibian species.

Original languageEnglish (US)
Pages (from-to)629-640
Number of pages12
JournalMolecular Reproduction and Development
Volume74
Issue number5
DOIs
StatePublished - May 1 2007
Externally publishedYes

Fingerprint

Bufo arenarum
Amphibians
Bufonidae
Oocytes
Spermatozoa
Glycoproteins
Ovum
Complementary DNA
Furin
Sperm-Ovum Interactions
Secretory Pathway
Biosynthetic Pathways
Xenopus laevis
Xenopus
Southern Blotting
Fertilization
Genes
Glass
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence

Keywords

  • Egg-envelope
  • Sperm-binding protein
  • ZPC isoforms

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Glycoproteins of the vitelline envelope of amphibian oocyte : Biological and molecular characterization of ZPC component (gp41) in Bufo arenarum. / Barisone, Gustavo; Krapf, Darío; Correa-Fiz, Florencia; Arranz, Silvia E.; Cabada, Marcelo O.

In: Molecular Reproduction and Development, Vol. 74, No. 5, 01.05.2007, p. 629-640.

Research output: Contribution to journalArticle

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