Glycoprotein secretion by tracheal explants cultured from rats exposed to ozone

Jerold A Last, M. D. Jennings, L. W. Schwartz, Carroll E Cross

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Tracheal explants from rats exposed to 0.8 ppm (1.9 mg per m3) of ozone 8 hours per day for 1 to 90 days were incubated in culture with glucosamine labeled with carbon-14 or hydrogen-3. Compared with tracheas from control rats exposed to filtered air, the explants demonstrated a decreased rate of glycoprotein secretion for exposure intervals of as long as one week, followed by a rebound to an increased rate of glycoprotein secretion for at least 12 weeks of continued exposure to ozone. Detailed study of the behavior of labeled glycoproteins from the culture medium on chromatography on columns of BioGel A-150m demonstrated that the ratio of the low to high molecular weight peaks increased when there was an increased rate of glycoprotein secretion. This is the first report of a direct biochemical effect induced by ozone on airway metabolism.

Original languageEnglish (US)
Pages (from-to)695-703
Number of pages9
JournalAmerican Review of Respiratory Disease
Volume116
Issue number4
StatePublished - 1977

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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