Glycoprotein expression in human milk during lactation

John W. Froehlich, Eric D. Dodds, Mariana Barboza Gardner, Erica L. McJimpsey, Richard R. Seipert, Jimi Francis, Hyun Joo An, Samara Freeman, J. Bruce German, Carlito B Lebrilla

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

While milk proteins have been studied for decades, strikingly little effort has been applied to determining how the post-translational modifications (PTMs) of these proteins may change during the course of lactation. PTMs, particularly glycosylation, can greatly influence protein structure, function, and stability and can particularly influence the gut where their degradation products are potentially bioactive. In this work, previously undiscovered temporal variations in both expression and glycosylation of the glycoproteome of human milk are observed. Lactoferrin, one of the most abundant glycoproteins in human milk, is shown to be dynamically glycosylated during the first 10 days of lactation. Variations in expression or glycosylation levels are also demonstrated for several other abundant whey proteins, including tenascin, bile salt-stimulated lipase, xanthine dehydrogenase, and mannose receptor.

Original languageEnglish (US)
Pages (from-to)6440-6448
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number10
DOIs
StatePublished - May 26 2010

Keywords

  • Breast milk
  • Glycoprotein
  • Lactation
  • Milk
  • Post-translational modification

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

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  • Cite this

    Froehlich, J. W., Dodds, E. D., Barboza Gardner, M., McJimpsey, E. L., Seipert, R. R., Francis, J., An, H. J., Freeman, S., German, J. B., & Lebrilla, C. B. (2010). Glycoprotein expression in human milk during lactation. Journal of Agricultural and Food Chemistry, 58(10), 6440-6448. https://doi.org/10.1021/jf100112x