Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain-specific, preferential consumption of glycans

Mariana Barboza Gardner, David A. Sela, Claire Pirim, Riccardo G. LoCascio, Samara L. Freeman, J. Bruce German, David A. Mills, Carlito B Lebrilla

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) without disaccharides and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We used a novel mass spectrometry approach involving matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated that there was preferential consumption of selected pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-through-put tool for comprehensive profiling of oligosaccharides in GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species.

Original languageEnglish (US)
Pages (from-to)7319-7325
Number of pages7
JournalApplied and Environmental Microbiology
Volume75
Issue number23
DOIs
StatePublished - Dec 2009

Fingerprint

galactooligosaccharides
Oligosaccharides
Fourier transform
Polysaccharides
Mass Spectrometry
desorption
ionization
polysaccharides
mass spectrometry
laser
Bifidobacterium
matrix
fermentation
ion
disaccharide
prebiotics
Cyclotrons
Prebiotics
monosaccharide
lasers

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain-specific, preferential consumption of glycans. / Barboza Gardner, Mariana; Sela, David A.; Pirim, Claire; LoCascio, Riccardo G.; Freeman, Samara L.; German, J. Bruce; Mills, David A.; Lebrilla, Carlito B.

In: Applied and Environmental Microbiology, Vol. 75, No. 23, 12.2009, p. 7319-7325.

Research output: Contribution to journalArticle

Barboza Gardner, Mariana ; Sela, David A. ; Pirim, Claire ; LoCascio, Riccardo G. ; Freeman, Samara L. ; German, J. Bruce ; Mills, David A. ; Lebrilla, Carlito B. / Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain-specific, preferential consumption of glycans. In: Applied and Environmental Microbiology. 2009 ; Vol. 75, No. 23. pp. 7319-7325.
@article{da779929f1724c8baef3a8ea5aabefd7,
title = "Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain-specific, preferential consumption of glycans",
abstract = "Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) without disaccharides and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We used a novel mass spectrometry approach involving matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated that there was preferential consumption of selected pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-through-put tool for comprehensive profiling of oligosaccharides in GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species.",
author = "{Barboza Gardner}, Mariana and Sela, {David A.} and Claire Pirim and LoCascio, {Riccardo G.} and Freeman, {Samara L.} and German, {J. Bruce} and Mills, {David A.} and Lebrilla, {Carlito B}",
year = "2009",
month = "12",
doi = "10.1128/AEM.00842-09",
language = "English (US)",
volume = "75",
pages = "7319--7325",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "23",

}

TY - JOUR

T1 - Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain-specific, preferential consumption of glycans

AU - Barboza Gardner, Mariana

AU - Sela, David A.

AU - Pirim, Claire

AU - LoCascio, Riccardo G.

AU - Freeman, Samara L.

AU - German, J. Bruce

AU - Mills, David A.

AU - Lebrilla, Carlito B

PY - 2009/12

Y1 - 2009/12

N2 - Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) without disaccharides and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We used a novel mass spectrometry approach involving matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated that there was preferential consumption of selected pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-through-put tool for comprehensive profiling of oligosaccharides in GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species.

AB - Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) without disaccharides and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We used a novel mass spectrometry approach involving matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated that there was preferential consumption of selected pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-through-put tool for comprehensive profiling of oligosaccharides in GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species.

UR - http://www.scopus.com/inward/record.url?scp=72949102869&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72949102869&partnerID=8YFLogxK

U2 - 10.1128/AEM.00842-09

DO - 10.1128/AEM.00842-09

M3 - Article

C2 - 19801485

AN - SCOPUS:72949102869

VL - 75

SP - 7319

EP - 7325

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 23

ER -