Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines

Maria Lorna A De Leoz, Hyun Joo An, Scott Kronewitter, Jaehan Kim, Sean Beecroft, Ruth Louise Vinall, Suzanne Miyamoto, Ralph W deVere White, Kit Lam, Carlito B Lebrilla

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.

Original languageEnglish (US)
Pages (from-to)243-258
Number of pages16
JournalDisease Markers
Volume25
Issue number4-5
StatePublished - 2008

Fingerprint

Glycomics
Biomarkers
Polysaccharides
Prostatic Neoplasms
Cells
Cell Line
Solid Phase Extraction
Serum
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Cyclotrons
Cyclotron resonance
Biopsy
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Androgen Receptors
Fourier Analysis
Prostate-Specific Antigen
Mannose
Ionization
Mass spectrometry
Prostate

Keywords

  • Biomarker
  • Glycans
  • Mass spectrometry
  • Prostate cancer
  • Serum

ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry
  • Molecular Biology
  • Genetics

Cite this

De Leoz, M. L. A., An, H. J., Kronewitter, S., Kim, J., Beecroft, S., Vinall, R. L., ... Lebrilla, C. B. (2008). Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines. Disease Markers, 25(4-5), 243-258.

Glycomic approach for potential biomarkers on prostate cancer : Profiling of N-linked glycans in human sera and pRNS cell lines. / De Leoz, Maria Lorna A; An, Hyun Joo; Kronewitter, Scott; Kim, Jaehan; Beecroft, Sean; Vinall, Ruth Louise; Miyamoto, Suzanne; deVere White, Ralph W; Lam, Kit; Lebrilla, Carlito B.

In: Disease Markers, Vol. 25, No. 4-5, 2008, p. 243-258.

Research output: Contribution to journalArticle

De Leoz, MLA, An, HJ, Kronewitter, S, Kim, J, Beecroft, S, Vinall, RL, Miyamoto, S, deVere White, RW, Lam, K & Lebrilla, CB 2008, 'Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines', Disease Markers, vol. 25, no. 4-5, pp. 243-258.
De Leoz MLA, An HJ, Kronewitter S, Kim J, Beecroft S, Vinall RL et al. Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines. Disease Markers. 2008;25(4-5):243-258.
De Leoz, Maria Lorna A ; An, Hyun Joo ; Kronewitter, Scott ; Kim, Jaehan ; Beecroft, Sean ; Vinall, Ruth Louise ; Miyamoto, Suzanne ; deVere White, Ralph W ; Lam, Kit ; Lebrilla, Carlito B. / Glycomic approach for potential biomarkers on prostate cancer : Profiling of N-linked glycans in human sera and pRNS cell lines. In: Disease Markers. 2008 ; Vol. 25, No. 4-5. pp. 243-258.
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