Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines

Maria Lorna A De Leoz, Hyun Joo An, Scott Kronewitter, Jaehan Kim, Sean Beecroft, Ruth Louise Vinall, Suzanne Miyamoto, Ralph W deVere White, Kit Lam, Carlito B Lebrilla

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.

Original languageEnglish (US)
Pages (from-to)243-258
Number of pages16
JournalDisease Markers
Volume25
Issue number4-5
StatePublished - 2008

Keywords

  • Biomarker
  • Glycans
  • Mass spectrometry
  • Prostate cancer
  • Serum

ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry
  • Molecular Biology
  • Genetics

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