Glycero- versus sphingo-phospholipids: Correlations with human and non-human mammalian lens growth

M. Cecilia Yappert, Madalina Rujoi, Douglas Borchman, Igor Vorobyov, Rosendo Estrada

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The human lens differs from other mammalian lenses in its very slow growth and unusual phospholipid composition of its cell membranes. Dihydrosphingomyelins (DHSMs) make up about half of all phospholipids in adult human fiber membranes. In all other membranes, sphingomyelins(SMs) with a trans double bond in their backbone, are prevalent. In our quest to understand the biological implications of such elevated DHSM levels, we analyzed membranes from various regions of human, elephant, giraffe, polar bear, pig and cow lenses. The levels of DHSMs were minor in non-human lens membranes. A strong correlation was observed between growth rate and relative contents of phosphatidylcholines(PCs) in epithelia and outer cortical fibers. Sphingomyelins became increasingly predominant in differentiated fibers and this increase was age dependent. Indeed, nuclear fiber membranes of aged non-human mammals were composed, almost exclusively, of (SMs). Although human lens membranes followed comparable compositional trends, the magnitude of the changes was much smaller. We postulate that the high relative contents of DHSMs provide a biochemically inert matrix in which only small amounts of PCs and SMs and their metabolites, known to promote and arrest growth, respectively, are present. This compositional difference is proposed to contribute to the slow multiplication and elongation of human lens cells.

Original languageEnglish (US)
Pages (from-to)725-734
Number of pages10
JournalExperimental Eye Research
Volume76
Issue number6
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

Fingerprint

Lenses
Phospholipids
Sphingomyelins
Growth
Membranes
Phosphatidylcholines
Ursidae
Nuclear Envelope
Mammals
Swine
Epithelium
Cell Membrane

Keywords

  • Age dependence
  • Dihydrosphingomyelin
  • Fiber membranes
  • Glycerolipids
  • Human lens
  • Lens growth
  • Mammalian lens
  • Phospholipids
  • Sphingolipids

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Glycero- versus sphingo-phospholipids : Correlations with human and non-human mammalian lens growth. / Yappert, M. Cecilia; Rujoi, Madalina; Borchman, Douglas; Vorobyov, Igor; Estrada, Rosendo.

In: Experimental Eye Research, Vol. 76, No. 6, 01.06.2003, p. 725-734.

Research output: Contribution to journalArticle

Yappert, M. Cecilia ; Rujoi, Madalina ; Borchman, Douglas ; Vorobyov, Igor ; Estrada, Rosendo. / Glycero- versus sphingo-phospholipids : Correlations with human and non-human mammalian lens growth. In: Experimental Eye Research. 2003 ; Vol. 76, No. 6. pp. 725-734.
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