Abstract
Naive T cells (CD45RA+) are selectively recruited to peripheral lymph nodes during lymphocyte recirculation. In this multistep process, L-selectin, a member of the family of carbohydrate-binding receptors involved in leukocyte-endothelial interactions, mediates the attachment oflymphocytes to high endothelial venules (HEV) in lymph nodes. In contrast to other HEV-associated ligands for L-selectin such as CD34 and MAdCAM-I, GlyCAM-1 is secreted without any apparent association with the vascular luminal surface. These observations suggest that GlyCAM-1 may perform a signaling role rather than a direct pro-adhesive function. We report here that either antibody-mediated crosslinking of L-selectin on peripheral lymphocytes or treatment of these cells with purified GlyCAM-1 stimulates their binding to ICAM-1 via beta 2 integrins. Furthermore, GlyCAM-1 causes the rapid expression (within seconds) of the mab24-defined neoepitope on beta 2 integrins that is associated with a high-avidity binding state. Strikingly, the naive population but not the memory population (CD45RO+) oflymphocytes responds to either crosslinking L-selectin or GlyCAM-I treatment. These results suggest that ligation of L-selectin by GlyCAM-1 or other ligands may provide key signals to the lymphocyte leading to the selective recruitment of naive cells into peripheral lymph nodes.
| Original language | English (US) |
|---|---|
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 6 |
| State | Published - 1996 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
GlyCAM-1, a physiologic ligand for l-selectin, activates beta 2 integrins in naive T lymphocytes. / Hwang, Samuel T; Simon, S. I.; Singer, M. S.; Giblin, P. A.; Rosen, S. D.
In: FASEB Journal, Vol. 10, No. 6, 1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - GlyCAM-1, a physiologic ligand for l-selectin, activates beta 2 integrins in naive T lymphocytes
AU - Hwang, Samuel T
AU - Simon, S. I.
AU - Singer, M. S.
AU - Giblin, P. A.
AU - Rosen, S. D.
PY - 1996
Y1 - 1996
N2 - Naive T cells (CD45RA+) are selectively recruited to peripheral lymph nodes during lymphocyte recirculation. In this multistep process, L-selectin, a member of the family of carbohydrate-binding receptors involved in leukocyte-endothelial interactions, mediates the attachment oflymphocytes to high endothelial venules (HEV) in lymph nodes. In contrast to other HEV-associated ligands for L-selectin such as CD34 and MAdCAM-I, GlyCAM-1 is secreted without any apparent association with the vascular luminal surface. These observations suggest that GlyCAM-1 may perform a signaling role rather than a direct pro-adhesive function. We report here that either antibody-mediated crosslinking of L-selectin on peripheral lymphocytes or treatment of these cells with purified GlyCAM-1 stimulates their binding to ICAM-1 via beta 2 integrins. Furthermore, GlyCAM-1 causes the rapid expression (within seconds) of the mab24-defined neoepitope on beta 2 integrins that is associated with a high-avidity binding state. Strikingly, the naive population but not the memory population (CD45RO+) oflymphocytes responds to either crosslinking L-selectin or GlyCAM-I treatment. These results suggest that ligation of L-selectin by GlyCAM-1 or other ligands may provide key signals to the lymphocyte leading to the selective recruitment of naive cells into peripheral lymph nodes.
AB - Naive T cells (CD45RA+) are selectively recruited to peripheral lymph nodes during lymphocyte recirculation. In this multistep process, L-selectin, a member of the family of carbohydrate-binding receptors involved in leukocyte-endothelial interactions, mediates the attachment oflymphocytes to high endothelial venules (HEV) in lymph nodes. In contrast to other HEV-associated ligands for L-selectin such as CD34 and MAdCAM-I, GlyCAM-1 is secreted without any apparent association with the vascular luminal surface. These observations suggest that GlyCAM-1 may perform a signaling role rather than a direct pro-adhesive function. We report here that either antibody-mediated crosslinking of L-selectin on peripheral lymphocytes or treatment of these cells with purified GlyCAM-1 stimulates their binding to ICAM-1 via beta 2 integrins. Furthermore, GlyCAM-1 causes the rapid expression (within seconds) of the mab24-defined neoepitope on beta 2 integrins that is associated with a high-avidity binding state. Strikingly, the naive population but not the memory population (CD45RO+) oflymphocytes responds to either crosslinking L-selectin or GlyCAM-I treatment. These results suggest that ligation of L-selectin by GlyCAM-1 or other ligands may provide key signals to the lymphocyte leading to the selective recruitment of naive cells into peripheral lymph nodes.
UR - http://www.scopus.com/inward/record.url?scp=33749095382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749095382&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33749095382
VL - 10
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 6
ER -