GlyCAM-1, a physiologic ligand for l-selectin, activates beta 2 integrins in naive T lymphocytes

Samuel T Hwang, S. I. Simon, M. S. Singer, P. A. Giblin, S. D. Rosen

Research output: Contribution to journalArticle

Abstract

Naive T cells (CD45RA+) are selectively recruited to peripheral lymph nodes during lymphocyte recirculation. In this multistep process, L-selectin, a member of the family of carbohydrate-binding receptors involved in leukocyte-endothelial interactions, mediates the attachment oflymphocytes to high endothelial venules (HEV) in lymph nodes. In contrast to other HEV-associated ligands for L-selectin such as CD34 and MAdCAM-I, GlyCAM-1 is secreted without any apparent association with the vascular luminal surface. These observations suggest that GlyCAM-1 may perform a signaling role rather than a direct pro-adhesive function. We report here that either antibody-mediated crosslinking of L-selectin on peripheral lymphocytes or treatment of these cells with purified GlyCAM-1 stimulates their binding to ICAM-1 via beta 2 integrins. Furthermore, GlyCAM-1 causes the rapid expression (within seconds) of the mab24-defined neoepitope on beta 2 integrins that is associated with a high-avidity binding state. Strikingly, the naive population but not the memory population (CD45RO+) oflymphocytes responds to either crosslinking L-selectin or GlyCAM-I treatment. These results suggest that ligation of L-selectin by GlyCAM-1 or other ligands may provide key signals to the lymphocyte leading to the selective recruitment of naive cells into peripheral lymph nodes.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996
Externally publishedYes

Fingerprint

Integrin beta Chains
L-Selectin
Selectins
T-cells
integrins
Integrins
lymph nodes
lymphocytes
T-lymphocytes
Lymphocytes
Ligands
T-Lymphocytes
crosslinking
Venules
Lymph Nodes
carbohydrate binding
Crosslinking
blood vessels
adhesives
leukocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

GlyCAM-1, a physiologic ligand for l-selectin, activates beta 2 integrins in naive T lymphocytes. / Hwang, Samuel T; Simon, S. I.; Singer, M. S.; Giblin, P. A.; Rosen, S. D.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Hwang, Samuel T ; Simon, S. I. ; Singer, M. S. ; Giblin, P. A. ; Rosen, S. D. / GlyCAM-1, a physiologic ligand for l-selectin, activates beta 2 integrins in naive T lymphocytes. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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AU - Hwang, Samuel T

AU - Simon, S. I.

AU - Singer, M. S.

AU - Giblin, P. A.

AU - Rosen, S. D.

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AB - Naive T cells (CD45RA+) are selectively recruited to peripheral lymph nodes during lymphocyte recirculation. In this multistep process, L-selectin, a member of the family of carbohydrate-binding receptors involved in leukocyte-endothelial interactions, mediates the attachment oflymphocytes to high endothelial venules (HEV) in lymph nodes. In contrast to other HEV-associated ligands for L-selectin such as CD34 and MAdCAM-I, GlyCAM-1 is secreted without any apparent association with the vascular luminal surface. These observations suggest that GlyCAM-1 may perform a signaling role rather than a direct pro-adhesive function. We report here that either antibody-mediated crosslinking of L-selectin on peripheral lymphocytes or treatment of these cells with purified GlyCAM-1 stimulates their binding to ICAM-1 via beta 2 integrins. Furthermore, GlyCAM-1 causes the rapid expression (within seconds) of the mab24-defined neoepitope on beta 2 integrins that is associated with a high-avidity binding state. Strikingly, the naive population but not the memory population (CD45RO+) oflymphocytes responds to either crosslinking L-selectin or GlyCAM-I treatment. These results suggest that ligation of L-selectin by GlyCAM-1 or other ligands may provide key signals to the lymphocyte leading to the selective recruitment of naive cells into peripheral lymph nodes.

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