TY - JOUR
T1 - GlyCAM-1, a physiologic ligand for L-selectin, activates β2 integrins on naive peripheral lymphocytes
AU - Hwang, Sam T.
AU - Singer, Mark S.
AU - Giblin, Patricia A.
AU - Yednock, Ted A.
AU - Bacon, Kevin B.
AU - Simon, Scott I
AU - Rosen, Steven D.
PY - 1996/10/1
Y1 - 1996/10/1
N2 - Naive T cells are selectively recruited from the blood into peripheral lymph nodes during lymphocyte recirculation. L-selectin, a lectin-like receptor, mediates the initial attachment of lymphocytes to high endothelial venules (HEV) in lymph nodes. A subsequent step involving the activation of β2 integrins has been proposed to facilitate firm adhesion, but the activating signals are poorly understood. We report here that here that either antibody-mediated cross-linking of L-selectin on human lymphocytes or treatment of the cells with GlyCAM-1, an HEV-derived, secreted ligand for L- selectin, stimulates their binding to ICAM-1 through the β2 integrin pathway. Furthermore, GlyCAm-1 causes the rapid expression of a neoepitope on β2 integrins associated with a high-avidity state. Naive (CD45RA+), but not memory (CD45RO+) lymphocytes, respond to L-selectin cross-linking or GlyCAM- 1 treatment. Thus, the complexing of L-selectin by specific ligands may provide key signals to naive lymphocytes, contributing to their selective recruitment into peripheral lymphoid organs.
AB - Naive T cells are selectively recruited from the blood into peripheral lymph nodes during lymphocyte recirculation. L-selectin, a lectin-like receptor, mediates the initial attachment of lymphocytes to high endothelial venules (HEV) in lymph nodes. A subsequent step involving the activation of β2 integrins has been proposed to facilitate firm adhesion, but the activating signals are poorly understood. We report here that here that either antibody-mediated cross-linking of L-selectin on human lymphocytes or treatment of the cells with GlyCAM-1, an HEV-derived, secreted ligand for L- selectin, stimulates their binding to ICAM-1 through the β2 integrin pathway. Furthermore, GlyCAm-1 causes the rapid expression of a neoepitope on β2 integrins associated with a high-avidity state. Naive (CD45RA+), but not memory (CD45RO+) lymphocytes, respond to L-selectin cross-linking or GlyCAM- 1 treatment. Thus, the complexing of L-selectin by specific ligands may provide key signals to naive lymphocytes, contributing to their selective recruitment into peripheral lymphoid organs.
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U2 - 10.1084/jem.184.4.1343
DO - 10.1084/jem.184.4.1343
M3 - Article
C2 - 8879206
AN - SCOPUS:0029904693
VL - 184
SP - 1343
EP - 1348
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 4
ER -