Glutamate receptor blockade attenuates glucose hypermetabolism in perihematomal brain after experimental intracerebral hemorrhage in rat

Timothy D. Ardizzone, Aigang Lu, Kenneth R. Wagner, Yang Tang, Ruiqiong Ran, Frank R Sharp

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background and Purpose - Intracerebral hemorrhage has no effective treatment. The delayed appearance of edema, apoptosis, and inflammation in perihematomal brain suggests that these events may be targets for therapeutic intervention. To develop successful treatments, we must learn more about the effects of hemorrhage on brain tissue. In this study, we investigated the acute metabolic effects of intrastriatal hemorrhage in rat brain. Methods - Lysed blood or saline (50 μL each) was injected into the striatum of male Sprague-Dawley rats. The rats recovered for 1 to 72 hours before injection of [14C]-2-deoxyglucose (intraperitoneally) 30 minutes before decapitation. Animals were pretreated with the N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) glutamate receptor antagonists dizolcilpine maleate (MK-801; 1 mg/kg) or 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo[f]quinoxaline (NBQX; 30 mg/kg), or saline vehicle. Additional animals received intrastriatal injections of glutamate (1.0 mmol/L), NMDA (1.0 mmol/L), or AMPA (0.1 mmol/L) in the place of blood. Semiquantitative autoradiographs from the brains were analyzed to determine the effects of hemorrhage on relative glucose metabolism. Results - We found an acute phase of increased [14C]-2-deoxyglucose uptake in the perihematomal region that peaks 3 hours after lysed blood injection. Saline injections had no effect on striatal glucose utilization. The increased [ 14C]-2-deoxyglucose uptake produced by the hemorrhages was blocked by pretreatment with MK-801 and NBQX. Glutamate injections alone had no effect on striatal metabolism, whereas NMDA and AMPA injections increased [ 14C]-2-deoxyglucose uptake. Conclusions - The data imply that glutamate activation of NMDA or AMPA receptors increases glucose metabolism in perihematomal brain at early times after intracerebral hemorrhage. This may provide a possible target for the treatment of intracerebral hemorrhage.

Original languageEnglish (US)
Pages (from-to)2587-2591
Number of pages5
JournalStroke
Volume35
Issue number11
DOIs
StatePublished - Nov 2004

Fingerprint

Cerebral Hemorrhage
Glutamate Receptors
Deoxyglucose
Glucose
Injections
Brain
N-Methylaspartate
Corpus Striatum
Glutamic Acid
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
Dizocilpine Maleate
Hemorrhage
Decapitation
Quinoxalines
Excitatory Amino Acid Antagonists
Intracranial Hemorrhages
Therapeutics
N-Methyl-D-Aspartate Receptors
Sprague Dawley Rats

Keywords

  • Hemorrhagic
  • Intracerebral hemorrhage
  • MK-801
  • Stroke

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Glutamate receptor blockade attenuates glucose hypermetabolism in perihematomal brain after experimental intracerebral hemorrhage in rat. / Ardizzone, Timothy D.; Lu, Aigang; Wagner, Kenneth R.; Tang, Yang; Ran, Ruiqiong; Sharp, Frank R.

In: Stroke, Vol. 35, No. 11, 11.2004, p. 2587-2591.

Research output: Contribution to journalArticle

Ardizzone, Timothy D. ; Lu, Aigang ; Wagner, Kenneth R. ; Tang, Yang ; Ran, Ruiqiong ; Sharp, Frank R. / Glutamate receptor blockade attenuates glucose hypermetabolism in perihematomal brain after experimental intracerebral hemorrhage in rat. In: Stroke. 2004 ; Vol. 35, No. 11. pp. 2587-2591.
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