Glutamate increases glycogen content and reduces glucose utilization in primary astrocyte culture

Raymond A. Swanson, Albert C H Yu, Pak H. Chan, Frank R Sharp

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The glycogen content of primary cultured astrocytes was approximately doubled by incubation with 1 mM L-glutamate or L-aspartate. Other amino acids and excitatory neurotransmitters were without effect. The increase in glycogen level was not blocked by the glutamate receptor antagonist kynurenic acid but was completely blocked by the glutamate uptake inhibitor threo-3-hydroxy-D,L-aspartate and by removal of Na+ from the medium. Incubation with radiolabeled glucose and glutamate revealed that the increased glycogen content was derived almost entirely from glucose. Glutamate at 1 mM was also found to cause a 53 ± 12% decrease in glucose utilization and a 112 ± 69% increase in glucose-6-phosphate levels. These results suggest that the glycogen content of astrocytes is linked to the rate of glucose utilization and that glucose utilization can, in turn, be affected by the availability of alternative metabolic substrates. These relationships suggest a mechanism by which brain glycogen accumulation occurs during decreased neuronal activity.

Original languageEnglish (US)
Pages (from-to)490-496
Number of pages7
JournalJournal of Neurochemistry
Volume54
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Glycogen
Astrocytes
Glutamic Acid
Glucose
Aspartic Acid
Kynurenic Acid
D-Aspartic Acid
Excitatory Amino Acid Antagonists
Glucose-6-Phosphate
Excitatory Amino Acids
Neurotransmitter Agents
Brain
Availability
Substrates

Keywords

  • 2-Deoxyglucose
  • Aspartate
  • Excitatory amino acid
  • Glia
  • Glucose-6-phosphate
  • Methionine sulfoxi-mine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Glutamate increases glycogen content and reduces glucose utilization in primary astrocyte culture. / Swanson, Raymond A.; Yu, Albert C H; Chan, Pak H.; Sharp, Frank R.

In: Journal of Neurochemistry, Vol. 54, No. 2, 1990, p. 490-496.

Research output: Contribution to journalArticle

Swanson, Raymond A. ; Yu, Albert C H ; Chan, Pak H. ; Sharp, Frank R. / Glutamate increases glycogen content and reduces glucose utilization in primary astrocyte culture. In: Journal of Neurochemistry. 1990 ; Vol. 54, No. 2. pp. 490-496.
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