GluR5 kainate receptor mediated synaptic transmission in rat basolateral amygdala in vitro

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

A non-NMDA and non-AMPA receptor mediated excitatory synaptic response was identified in intracellularly recorded basolateral amygdala (BLA) neurons in an in vitro slice preparation. Synaptic potentials were evoked by stimulation of either the external capsule (EC) or basal amygdala (BA). NMDA and GABA(A) receptors were blocked by inclusion of 100 μM (±)-2-amino-5-phosphonopentanoic acid and 10 μM bicuculline in the perfusion solution. The AMPA receptor-selective allosteric antagonists GYKI 52466 (50 μM) and GYKI 53655 (50 μM) partially suppressed depolarizing synaptic responses evoked by single shock EC stimulation, but fully blocked synaptic responses evoked by BA stimulation. In recordings carried out in the presence of the AMPA receptor antagonists, EC stimulation with pulse trains (5-8 pulses at 50-100 Hz) evoked a large increase in the amplitude of synaptic responses. The AMPA receptor-independent component of the train-induced synaptic response had a null potential near 0 mV. Such AMPA receptor-independent, train-evoked synaptic responses were largely blocked by the AMPA/kainate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (20 μM; 85±4%). In addition, the responses were blocked by the GluR5-selective kainate receptor antagonist LY293558 (10 μM; 95±2%). These results indicate that a component of the EC (but not the BA) synaptic response is mediated by kainate receptors containing the GluR5 subunit. Copyright (C) 1998 Published by Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)1279-1286
Number of pages8
JournalNeuropharmacology
Volume37
Issue number10-11
DOIs
StatePublished - Oct 1 1998
Externally publishedYes

Fingerprint

AMPA Receptors
Synaptic Transmission
Amygdala
GYKI 53655
tezampanel
6-Cyano-7-nitroquinoxaline-2,3-dione
2-Amino-5-phosphonovalerate
Kainic Acid Receptors
Synaptic Potentials
Bicuculline
N-Methylaspartate
GABA-A Receptors
Shock
Perfusion
Basolateral Nuclear Complex
Gluk1 kainate receptor
In Vitro Techniques
Neurons
External Capsule

Keywords

  • 2,3-Benzodiazepine
  • 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX)
  • Amygdala
  • Kainate receptor
  • LY293558
  • Synaptic transmission

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

GluR5 kainate receptor mediated synaptic transmission in rat basolateral amygdala in vitro. / Li, He; Rogawski, Michael A.

In: Neuropharmacology, Vol. 37, No. 10-11, 01.10.1998, p. 1279-1286.

Research output: Contribution to journalArticle

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