Abstract
Tissue glucose transporter (GLUT1-3) and cationic amino acid transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on days 1, 3 and 7 or days 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on day 1. All tissues expressed a GLUT isoform on day 7 except for the thymus. Most tissues expressing a CAT isoform on day 1 decreased mRNA levels through day 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05). The thymus and spleen did not express any CAT isoform mRNA until day 7. The liver was the only tissue expressing GLUT-2 mRNA through day 14. On day 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05). High-affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively (P<0.05). Total CAT mRNA expression was greatest in the bursa (P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on day 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 515-525 |
| Number of pages | 11 |
| Journal | Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology |
| Volume | 138 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2004 |
Fingerprint
Keywords
- Arginine
- Glucose
- Growth
- Lymphocyte
- Lysine
- Ontogeny
- Transport
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Physiology
Cite this
Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus). / Humphrey, Brooke D.; Stephensen, Charles B.; Calvert, Chris C.; Klasing, Kirk C.
In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 138, No. 4, 08.2004, p. 515-525.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus)
AU - Humphrey, Brooke D.
AU - Stephensen, Charles B.
AU - Calvert, Chris C.
AU - Klasing, Kirk C.
PY - 2004/8
Y1 - 2004/8
N2 - Tissue glucose transporter (GLUT1-3) and cationic amino acid transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on days 1, 3 and 7 or days 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on day 1. All tissues expressed a GLUT isoform on day 7 except for the thymus. Most tissues expressing a CAT isoform on day 1 decreased mRNA levels through day 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05). The thymus and spleen did not express any CAT isoform mRNA until day 7. The liver was the only tissue expressing GLUT-2 mRNA through day 14. On day 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05). High-affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively (P<0.05). Total CAT mRNA expression was greatest in the bursa (P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on day 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.
AB - Tissue glucose transporter (GLUT1-3) and cationic amino acid transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on days 1, 3 and 7 or days 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on day 1. All tissues expressed a GLUT isoform on day 7 except for the thymus. Most tissues expressing a CAT isoform on day 1 decreased mRNA levels through day 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05). The thymus and spleen did not express any CAT isoform mRNA until day 7. The liver was the only tissue expressing GLUT-2 mRNA through day 14. On day 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05). High-affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively (P<0.05). Total CAT mRNA expression was greatest in the bursa (P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on day 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.
KW - Arginine
KW - Glucose
KW - Growth
KW - Lymphocyte
KW - Lysine
KW - Ontogeny
KW - Transport
UR - http://www.scopus.com/inward/record.url?scp=4644369773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4644369773&partnerID=8YFLogxK
U2 - 10.1016/j.cbpb.2004.06.016
DO - 10.1016/j.cbpb.2004.06.016
M3 - Article
C2 - 15369841
AN - SCOPUS:4644369773
VL - 138
SP - 515
EP - 525
JO - Comparative Biochemistry and Physiology - A Physiology
JF - Comparative Biochemistry and Physiology - A Physiology
SN - 1095-6433
IS - 4
ER -