Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus)

Brooke D. Humphrey, Charles B. Stephensen, Chris C. Calvert, Kirk C. Klasing

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Tissue glucose transporter (GLUT1-3) and cationic amino acid transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on days 1, 3 and 7 or days 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on day 1. All tissues expressed a GLUT isoform on day 7 except for the thymus. Most tissues expressing a CAT isoform on day 1 decreased mRNA levels through day 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05). The thymus and spleen did not express any CAT isoform mRNA until day 7. The liver was the only tissue expressing GLUT-2 mRNA through day 14. On day 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05). High-affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively (P<0.05). Total CAT mRNA expression was greatest in the bursa (P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on day 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.

Original languageEnglish (US)
Pages (from-to)515-525
Number of pages11
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume138
Issue number4
DOIs
StatePublished - Aug 2004

Fingerprint

Basic Amino Acid Transport Systems
Chickens
Thymus
Tissue
Glucose
Messenger RNA
Thymus Gland
Protein Isoforms
Liver
RNA Isoforms
T-cells
Facilitative Glucose Transport Proteins
Spleen
T-Lymphocytes
Amino Acids

Keywords

  • Arginine
  • Glucose
  • Growth
  • Lymphocyte
  • Lysine
  • Ontogeny
  • Transport

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus). / Humphrey, Brooke D.; Stephensen, Charles B.; Calvert, Chris C.; Klasing, Kirk C.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 138, No. 4, 08.2004, p. 515-525.

Research output: Contribution to journalArticle

Humphrey, Brooke D. ; Stephensen, Charles B. ; Calvert, Chris C. ; Klasing, Kirk C. / Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus). In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 2004 ; Vol. 138, No. 4. pp. 515-525.
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