Global gene expression profiling of androgen disruption in Qurt strain medaka

Abimael León, Ping Shi Wu, Linda C. Hall, Michael L. Johnson, Swee J Teh

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Androgen disrupting chemicals (ADCs) are endocrine disrupting chemicals (EDCs) that mimic or antagonize the effect of physiological androgens. Microarray-based detection of altered gene expression can be used as a biomarker of EDC exposure. Therefore, the purpose of this study was to identify and compare gene expression profiles of the androgen 11-ketotestosterone (11-KT), the antiandrogen flutamide (FLU), and the antiandrogenic fungicide vinclozolin (VIN), on Qurt medaka (Oryzias latipes). Biologically effective concentrations for 11-KT (100 μg/L), VIN (100 μg/L), and FLU (1000 μg/L) determined in range-finding studies were used for exposures. The oligonucleotide microarray included 9379 probes for EDC-affected genes, medaka cDNAs, sequences from the medaka genome project and the UniGene database. We found that treatment with FLU, VIN, and 11-KT caused significant (false discovery rate = 0.01) differential expression of at least 87, 82, and 578 genes, respectively. Two sets of responsive genes are associated to vertebrate sex differentiation and growth, and 50 genes were useful in discriminating between ADC classes. The discriminating capacity was confirmed by a remarkable similarity of the antiandrogenic expression profiles of VIN and FLU, which were distinct from the androgenic profile of 11-KT. Gene expression profiles characterized in this study allow for reliable screening of ADC activity.

Original languageEnglish (US)
Pages (from-to)962-969
Number of pages8
JournalEnvironmental Science and Technology
Volume42
Issue number3
DOIs
StatePublished - Feb 1 2008

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ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

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