Global conformation of a self-cleaving hammerhead RNA

Khaled M A Amiri, Paul J Hagerman

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Phylogenetic and biochemical studies of RNA have provided a wealth of information regarding secondary structure; however, knowledge of tertiary structure has been more difficult to obtain. In the current work, electrophoretic and hydrodynamic measurements have yielded the global conformations of a self-cleaving "hammerhead" RNA, both prior to and following self-cleavage. The pre- - and post-cleavage structures appear to have nearly identical conformations in which the three helices assume a roughly coplanar arrangement with well-defined interhelix angles. Following self-cleavage, one of the three helix stems (stem I) undergoes a slight (<10°) realignment, although the relative positions of the other two stems remain unchanged. Extension of stem III from (nominally) three base pairs to nine base pairs, known to dramatically enhance the rate of self-cleavage, results in a substantial (∼70°) realignment of stems I and II. The current study represents an approach for studying other nonhelical elements in RNA.

Original languageEnglish (US)
Pages (from-to)13172-13177
Number of pages6
JournalBiochemistry®
Volume33
Issue number45
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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