Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters

Yi Cao, Roshan M. Kumar, Bennett Penn, Charlotte A. Berkes, Charles Kooperberg, Laurie A. Boyer, Richard A. Young, Stephen J. Tapscott

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

We used a combination of genome-wide and promoter-specific DNA binding and expression analyses to assess the functional roles of Myod and Myog in regulating the program of skeletal muscle gene expression. Our findings indicate that Myod and Myog have distinct regulatory roles at a similar set of target genes. At genes expressed throughout the program of myogenic differentiation, Myod can bind and recruit histone acetyltransferases. At early targets, Myod is sufficient for near full expression, whereas, at late expressed genes, Myod initiates regional histone modification but is not sufficient for gene expression. At these late genes, Myog does not bind efficiently without Myod; however, transcriptional activation requires the combined activity of Myod and Myog. Therefore, the role of Myog in mediating terminal differentiation is, in part, to enhance expression of a subset of genes previously initiated by Myod.

Original languageEnglish (US)
Pages (from-to)502-511
Number of pages10
JournalEMBO Journal
Volume25
Issue number3
DOIs
StatePublished - Feb 8 2006
Externally publishedYes

Fingerprint

Genes
Gene expression
Histone Code
Histone Acetyltransferases
Gene Expression
Transcriptional Activation
Skeletal Muscle
Histones
Muscle
Genome
Chemical activation
DNA

Keywords

  • Muscle differentiation
  • Myod
  • Myogenin
  • Regulatory network

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Cao, Y., Kumar, R. M., Penn, B., Berkes, C. A., Kooperberg, C., Boyer, L. A., ... Tapscott, S. J. (2006). Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters. EMBO Journal, 25(3), 502-511. https://doi.org/10.1038/sj.emboj.7600958

Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters. / Cao, Yi; Kumar, Roshan M.; Penn, Bennett; Berkes, Charlotte A.; Kooperberg, Charles; Boyer, Laurie A.; Young, Richard A.; Tapscott, Stephen J.

In: EMBO Journal, Vol. 25, No. 3, 08.02.2006, p. 502-511.

Research output: Contribution to journalArticle

Cao, Y, Kumar, RM, Penn, B, Berkes, CA, Kooperberg, C, Boyer, LA, Young, RA & Tapscott, SJ 2006, 'Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters', EMBO Journal, vol. 25, no. 3, pp. 502-511. https://doi.org/10.1038/sj.emboj.7600958
Cao, Yi ; Kumar, Roshan M. ; Penn, Bennett ; Berkes, Charlotte A. ; Kooperberg, Charles ; Boyer, Laurie A. ; Young, Richard A. ; Tapscott, Stephen J. / Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters. In: EMBO Journal. 2006 ; Vol. 25, No. 3. pp. 502-511.
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