Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters

Yi Cao, Roshan M. Kumar, Bennett Penn, Charlotte A. Berkes, Charles Kooperberg, Laurie A. Boyer, Richard A. Young, Stephen J. Tapscott

Research output: Contribution to journalArticlepeer-review

191 Scopus citations


We used a combination of genome-wide and promoter-specific DNA binding and expression analyses to assess the functional roles of Myod and Myog in regulating the program of skeletal muscle gene expression. Our findings indicate that Myod and Myog have distinct regulatory roles at a similar set of target genes. At genes expressed throughout the program of myogenic differentiation, Myod can bind and recruit histone acetyltransferases. At early targets, Myod is sufficient for near full expression, whereas, at late expressed genes, Myod initiates regional histone modification but is not sufficient for gene expression. At these late genes, Myog does not bind efficiently without Myod; however, transcriptional activation requires the combined activity of Myod and Myog. Therefore, the role of Myog in mediating terminal differentiation is, in part, to enhance expression of a subset of genes previously initiated by Myod.

Original languageEnglish (US)
Pages (from-to)502-511
Number of pages10
JournalEMBO Journal
Issue number3
StatePublished - Feb 8 2006
Externally publishedYes


  • Muscle differentiation
  • Myod
  • Myogenin
  • Regulatory network

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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