Global analysis of arterial fluorescence decay spectra

Jean Michel I Maarek, Warren S. Grundfest, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingChapter


Global analysis of time-resolved fluorescence measured at multiple emission wavelengths was applied to simulated fluorescence spectra and arterial fluorescence spectra. Fluorescence of human aortic samples was produced with nitrogen laser excitation. Simulated spectra had decay characteristics in the range expected from previous studies of artery tissue. For both types of spectra, the emission decay was analyzed with global analysis to model the decay with a sum of exponentials. Decay constants were held fixed across wavelengths while pre-exponential coefficients were wavelength-dependent. For the simulated spectra, global analysis was compared to the traditional method in which decay constants and pre-exponential coefficients are assumed wavelength-dependent. On the simulated data, three decays could be reliably estimated by global analysis even when only two exponential decays were identified with the traditional method. On the arterial data, the intermediate decay and the long decay significantly increased between normal samples and fibrous plaque (1.7 to 2.5 ns; 7.0 to 8.4 ns). The pre-exponential coefficient of the long decay was larger in the blue range of the spectrum for the samples with advanced atherosclerosis. We conclude that global analysis markedly improves the recovery of exponential decay trends in time-resolved fluorescence spectra. Application to artery tissue fluorescence reveals characteristic spectral changes associated with atherosclerosis.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Number of pages10
StatePublished - 2000
Externally publishedYes
EventOptical Biopsy III - San Jose, CA, USA
Duration: Jan 23 2000Jan 24 2000


OtherOptical Biopsy III
CitySan Jose, CA, USA

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics


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