Glial growth factor-2 promotes the survival, migration and bromodeoxyuridine incorporation of mammalian neural crest cells in caudal neural tube explant cultures

Peter G. Bannerman, Susan Puhalla, Anuraag Sahai, Angela Shieh, Marie Berman, David E Pleasure

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Using an in vitro assay system, we found that GGF-2 increases the number of nascent trunk neural crest cells (NCC) present in the dorsal outgrowth derived from E12 caudal neural tube explants. Data is presented which suggests that this increased outgrowth was due to a combination of GGF-2 mediated effects, including its ability to promote (A) NCC survival by decreasing the percentage of NCC that undergo cell death via a mechanism involving DNA fragmentation, (B) the initial phases of NCC migration, (C) mitosis of peripherally migrating NCC. We also show that GGF-2 can promote the long-term survival of NCC in the absence of the neural tube. An immunohistochemical analysis indicates that NCC express erbB-2 and erbB-4 neuregulin receptors. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalDevelopmental Brain Research
Volume124
Issue number1-2
DOIs
StatePublished - Nov 30 2000
Externally publishedYes

Keywords

  • Apoptosis
  • BrdU incorporation
  • DNA fragmentation
  • Glial growth factor
  • Migration
  • Neural crest cells

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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