Glia induce dendritic growth in cultured sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists

Pamela J Lein, Hiroko Nagasawa Beck, Vidya Chandrasekaran, Patrick J. Gallagher, Hui Ling Chen, Yuan Lin, Xin Guo, Paul L. Kaplan, Henri Tiedge, Dennis Higgins

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Dendritic growth in cultured sympathetic neurons requires specific trophic interactions. Previous studies have demonstrated that either coculture with glia or exposure to recombinant bone morphogenetic proteins (BMPs) is both necessary and sufficient to induce dendrite formation. These observations led us to test the hypothesis that BMPs mediate glial-induced dendritic growth. In situ hybridization and immunocytochemical studies indicate that the spatiotemporal expression of BMP5, -6, and -7 in rat superior cervical ganglia (SCG) is consistent with their proposed role in dendritogenesis. In vitro, both SCG glia and neurons were found to express BMP mRNA and protein when grown in the presence or absence of the other cell type. However, addition of ganglionic glia to cultured sympathetic neurons causes a marked increase in BMP proteins coincident with a significant decrease in follistatin and noggin. Functional assays indicate that glial-induced dendritic growth is significantly reduced by BMP7 antibodies and completely inhibited by exogenous noggin and follistatin. These data suggest that glia influence the rapid perinatal expansion of the dendritic arbor in sympathetic neurons by increasing BMP activity via modulation of the balance between BMPs and their antagonists.

Original languageEnglish (US)
Pages (from-to)10377-10387
Number of pages11
JournalJournal of Neuroscience
Volume22
Issue number23
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Bone Morphogenetic Proteins
Neuroglia
Neurons
Growth
Follistatin
Superior Cervical Ganglion
Dendrites
Coculture Techniques
Recombinant Proteins
In Situ Hybridization
Proteins
Messenger RNA
Antibodies

Keywords

  • BMP antagonists
  • BMPs
  • Dendrites
  • Follistatin
  • Neuron-glia interactions
  • Noggin
  • Sympathetic neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lein, P. J., Beck, H. N., Chandrasekaran, V., Gallagher, P. J., Chen, H. L., Lin, Y., ... Higgins, D. (2002). Glia induce dendritic growth in cultured sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists. Journal of Neuroscience, 22(23), 10377-10387.

Glia induce dendritic growth in cultured sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists. / Lein, Pamela J; Beck, Hiroko Nagasawa; Chandrasekaran, Vidya; Gallagher, Patrick J.; Chen, Hui Ling; Lin, Yuan; Guo, Xin; Kaplan, Paul L.; Tiedge, Henri; Higgins, Dennis.

In: Journal of Neuroscience, Vol. 22, No. 23, 01.12.2002, p. 10377-10387.

Research output: Contribution to journalArticle

Lein, PJ, Beck, HN, Chandrasekaran, V, Gallagher, PJ, Chen, HL, Lin, Y, Guo, X, Kaplan, PL, Tiedge, H & Higgins, D 2002, 'Glia induce dendritic growth in cultured sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists', Journal of Neuroscience, vol. 22, no. 23, pp. 10377-10387.
Lein, Pamela J ; Beck, Hiroko Nagasawa ; Chandrasekaran, Vidya ; Gallagher, Patrick J. ; Chen, Hui Ling ; Lin, Yuan ; Guo, Xin ; Kaplan, Paul L. ; Tiedge, Henri ; Higgins, Dennis. / Glia induce dendritic growth in cultured sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 23. pp. 10377-10387.
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