GLI1, CTNNB1 and NOTCH1 protein expression in a thymic epithelial malignancy tissue microarray

Jonathan Riess, Robert West, Michelle Dean, Alex C. Klimowicz, Joel W. Neal, Chuong Hoang, Heather A. Wakelee

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background/Aim: Thymic epithelial tumors (TET) are rare. Wingless and INT (WNT), NOTCH and sonic hedgehog pathway interactions between thymocytes and thymic stroma are important to thymus and T-cell development. We analyzed a thymoma tissue microarray (TMA) for glioma associated oncogene homolog 1 (GLI1), NOTCH1 and catenin (cadherinassociated protein, beta 1) (CTNNB1) expression as surrogate markers of sonic hedgehog, NOTCH and WNT pathway activity. Materials and Methods: GLI1, NOTCH1 and CTNNB1 expression were assayed in a tissue microarray of 68 TET and eight benign thymus by fluorescent immunohistochemistry (AQUA) as surrogates for activity of the sonic hedgehog, NOTCH and WNT pathways respectively. Results: No difference in tumor GLI1 (mean 201 vs. 211, p=0.31), CTNNB1 (mean 222 vs. 306, p=0.66) or NOTCH1 expression (mean 317 vs. 325, p=0.82) was noted between thymic tumor and benign thymus. Conclusion: No evidence for preferential expression of GLI1, NOTCH1 or CTNNB1 was noted. High-throughput immuno - fluorescence using AQUA technology can help overcome limitations of small sample size and tissue heterogeneity when analyzing protein expression in thymic tumors.

Original languageEnglish (US)
Pages (from-to)669-676
Number of pages8
JournalAnticancer Research
Volume35
Issue number2
StatePublished - Feb 1 2015

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Keywords

  • Beta-catenin
  • Immunohistochemistry
  • Notch
  • Sonic hedgehog
  • Thymoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Riess, J., West, R., Dean, M., Klimowicz, A. C., Neal, J. W., Hoang, C., & Wakelee, H. A. (2015). GLI1, CTNNB1 and NOTCH1 protein expression in a thymic epithelial malignancy tissue microarray. Anticancer Research, 35(2), 669-676.