Ginsenoside compound K suppresses the abnormal activation of T lymphocytes in mice with collagen-induced arthritis

Kang Kang Liu, Qingtong Wang, Si Min Yang, Jing Yu Chen, Hua Xun Wu, Wei Wei

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Aim: To investigate the anti-Arthritis and immunomodulatory activities of ginsenoside compound K (C-K) in mice with collagen-induced arthritis (CIA). Methods: DBA/1 mice with CIA were treated with C-K (28, 56 or 112 mgkg-1d-1, ig) or the positive control methotrexate (2 mg/kg, ig, every 3 d) for 34 d. Splenic T and B lymphocytes were positively isolated using anti-CD3-coated magnetic beads or a pan B cell isolation kit. T lymphocyte subsets, and CD28, T cell receptor (TCR), cytotoxic T lymphocyte-Associated antigen-4 (CTLA-4) and programmed death-1 (PD-1) expression in purified splenic T lymphocytes were analyzed using flow cytometry, Western blotting and laser confocal microscopy. Results: C-K treatment significantly ameliorated the pathologic manifestations of CIA mice, remarkably inhibited T lymphocyte proliferation, and marginally inhibited the proliferation of B lymphocytes. C-K treatment significantly suppressed TNF-? and anti-CII antibody levels, and increased IFN-? level in the joints of CIA mice, but did not alter IL-4 production. Treatment of CIA mice with C-K significantly decreased the percentages of activated T cells, co-stimulatory molecule-expressing T cells and effector memory T cells, and increased the frequencies of naive T cells and regulatory T cells. Furthermore, C-K treatment significantly decreased the expression of CD28 and TCR, whereas it increased the expression of CTLA-4 and PD-1 on T lymphocytes of CIA mice. Methotrexate treatment exerted comparable effects in all these experiments. Conclusion: C-K suppresses the progression of CIA through regulating TCR, CD28, CTLA-4 and PD-1 expression, thus inhibiting the abnormal activation and differentiation of T lymphocytes.

Original languageEnglish (US)
Pages (from-to)599-612
Number of pages14
JournalActa Pharmacologica Sinica
Volume35
Issue number5
DOIs
StatePublished - May 1 2014
Externally publishedYes

Keywords

  • arthritis
  • CD28
  • cytokine
  • cytotoxic T lymphocyteassociated antigen-4
  • ginseng
  • ginsenoside compound K
  • immunoregulator
  • programmed death-1
  • T lymphocyte

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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