Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua

Amber F. Roegner, Miles E. Daniels, Woutrina A. Smith, Nicole Gottdenker, Laura M. Schwartz, James Liu, Amanda Campbell, Christine V. Fiorello

Research output: Contribution to journalArticle

Abstract

Indigenous Mayangna and Miskitu inhabit Nicaragua’s remote Bosawás Biosphere Reserve, located in the North Caribbean Coast Autonomous Region. They are sedentary horticulturists who supplement their diet with wild game, hunting with the assistance of dogs. To test whether hunting dogs increased the risk of human exposure to protozoal zoonotic neglected tropical diseases (NTDs), we sampled dogs from three communities varying in population size and level of contact with other communities. We screened dog feces (n = 58) for Giardia and Cryptosporidium DNA and sera (n = 78) for Trypanosoma cruzi antibodies and DNA. Giardia DNA was detected in 22% (13/58) of samples; sequencing revealed the presence of both zoonotic genotypes (assemblages A and B) and dog-specific genotypes (assemblages C and D). Giardia shedding was associated with community and age. Older dogs and those in the two, more accessible communities had greater odds of shedding parasites. Seroprevalence of T. cruzi antibodies, indicating prior exposure, was 9% (7/78). These results contribute to the limited literature on NTDs in indigenous populations, and suggest hunting dogs can both serve as sentinels of environmental NTDs and pose zoonotic risk for their owners and communities.

Original languageEnglish (US)
Pages (from-to)512-522
Number of pages11
JournalEcoHealth
Volume16
Issue number3
DOIs
StatePublished - Sep 1 2019

Fingerprint

Nicaragua
Giardia
Trypanosoma cruzi
Dogs
Neglected Diseases
Infection
Zoonoses
hunting
DNA
antibody
genotype
Genotype
Cryptosporidium
indigenous population
Antibodies
Seroepidemiologic Studies
Population Density
exposure
dog
biosphere reserve

Keywords

  • Bosawás
  • Domestic dogs
  • Giardia
  • Indigenous health
  • Nicaragua
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Ecology
  • Health, Toxicology and Mutagenesis

Cite this

Roegner, A. F., Daniels, M. E., Smith, W. A., Gottdenker, N., Schwartz, L. M., Liu, J., ... Fiorello, C. V. (2019). Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua. EcoHealth, 16(3), 512-522. https://doi.org/10.1007/s10393-019-01434-2

Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua. / Roegner, Amber F.; Daniels, Miles E.; Smith, Woutrina A.; Gottdenker, Nicole; Schwartz, Laura M.; Liu, James; Campbell, Amanda; Fiorello, Christine V.

In: EcoHealth, Vol. 16, No. 3, 01.09.2019, p. 512-522.

Research output: Contribution to journalArticle

Roegner, AF, Daniels, ME, Smith, WA, Gottdenker, N, Schwartz, LM, Liu, J, Campbell, A & Fiorello, CV 2019, 'Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua', EcoHealth, vol. 16, no. 3, pp. 512-522. https://doi.org/10.1007/s10393-019-01434-2
Roegner, Amber F. ; Daniels, Miles E. ; Smith, Woutrina A. ; Gottdenker, Nicole ; Schwartz, Laura M. ; Liu, James ; Campbell, Amanda ; Fiorello, Christine V. / Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua. In: EcoHealth. 2019 ; Vol. 16, No. 3. pp. 512-522.
@article{73023f2b633c4069b6d8b26c44d9bc03,
title = "Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosaw{\'a}s Biosphere Reserve, Nicaragua",
abstract = "Indigenous Mayangna and Miskitu inhabit Nicaragua’s remote Bosaw{\'a}s Biosphere Reserve, located in the North Caribbean Coast Autonomous Region. They are sedentary horticulturists who supplement their diet with wild game, hunting with the assistance of dogs. To test whether hunting dogs increased the risk of human exposure to protozoal zoonotic neglected tropical diseases (NTDs), we sampled dogs from three communities varying in population size and level of contact with other communities. We screened dog feces (n = 58) for Giardia and Cryptosporidium DNA and sera (n = 78) for Trypanosoma cruzi antibodies and DNA. Giardia DNA was detected in 22{\%} (13/58) of samples; sequencing revealed the presence of both zoonotic genotypes (assemblages A and B) and dog-specific genotypes (assemblages C and D). Giardia shedding was associated with community and age. Older dogs and those in the two, more accessible communities had greater odds of shedding parasites. Seroprevalence of T. cruzi antibodies, indicating prior exposure, was 9{\%} (7/78). These results contribute to the limited literature on NTDs in indigenous populations, and suggest hunting dogs can both serve as sentinels of environmental NTDs and pose zoonotic risk for their owners and communities.",
keywords = "Bosaw{\'a}s, Domestic dogs, Giardia, Indigenous health, Nicaragua, Trypanosoma cruzi",
author = "Roegner, {Amber F.} and Daniels, {Miles E.} and Smith, {Woutrina A.} and Nicole Gottdenker and Schwartz, {Laura M.} and James Liu and Amanda Campbell and Fiorello, {Christine V.}",
year = "2019",
month = "9",
day = "1",
doi = "10.1007/s10393-019-01434-2",
language = "English (US)",
volume = "16",
pages = "512--522",
journal = "EcoHealth",
issn = "1612-9202",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua

AU - Roegner, Amber F.

AU - Daniels, Miles E.

AU - Smith, Woutrina A.

AU - Gottdenker, Nicole

AU - Schwartz, Laura M.

AU - Liu, James

AU - Campbell, Amanda

AU - Fiorello, Christine V.

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Indigenous Mayangna and Miskitu inhabit Nicaragua’s remote Bosawás Biosphere Reserve, located in the North Caribbean Coast Autonomous Region. They are sedentary horticulturists who supplement their diet with wild game, hunting with the assistance of dogs. To test whether hunting dogs increased the risk of human exposure to protozoal zoonotic neglected tropical diseases (NTDs), we sampled dogs from three communities varying in population size and level of contact with other communities. We screened dog feces (n = 58) for Giardia and Cryptosporidium DNA and sera (n = 78) for Trypanosoma cruzi antibodies and DNA. Giardia DNA was detected in 22% (13/58) of samples; sequencing revealed the presence of both zoonotic genotypes (assemblages A and B) and dog-specific genotypes (assemblages C and D). Giardia shedding was associated with community and age. Older dogs and those in the two, more accessible communities had greater odds of shedding parasites. Seroprevalence of T. cruzi antibodies, indicating prior exposure, was 9% (7/78). These results contribute to the limited literature on NTDs in indigenous populations, and suggest hunting dogs can both serve as sentinels of environmental NTDs and pose zoonotic risk for their owners and communities.

AB - Indigenous Mayangna and Miskitu inhabit Nicaragua’s remote Bosawás Biosphere Reserve, located in the North Caribbean Coast Autonomous Region. They are sedentary horticulturists who supplement their diet with wild game, hunting with the assistance of dogs. To test whether hunting dogs increased the risk of human exposure to protozoal zoonotic neglected tropical diseases (NTDs), we sampled dogs from three communities varying in population size and level of contact with other communities. We screened dog feces (n = 58) for Giardia and Cryptosporidium DNA and sera (n = 78) for Trypanosoma cruzi antibodies and DNA. Giardia DNA was detected in 22% (13/58) of samples; sequencing revealed the presence of both zoonotic genotypes (assemblages A and B) and dog-specific genotypes (assemblages C and D). Giardia shedding was associated with community and age. Older dogs and those in the two, more accessible communities had greater odds of shedding parasites. Seroprevalence of T. cruzi antibodies, indicating prior exposure, was 9% (7/78). These results contribute to the limited literature on NTDs in indigenous populations, and suggest hunting dogs can both serve as sentinels of environmental NTDs and pose zoonotic risk for their owners and communities.

KW - Bosawás

KW - Domestic dogs

KW - Giardia

KW - Indigenous health

KW - Nicaragua

KW - Trypanosoma cruzi

UR - http://www.scopus.com/inward/record.url?scp=85070935056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070935056&partnerID=8YFLogxK

U2 - 10.1007/s10393-019-01434-2

DO - 10.1007/s10393-019-01434-2

M3 - Article

C2 - 31414319

AN - SCOPUS:85070935056

VL - 16

SP - 512

EP - 522

JO - EcoHealth

JF - EcoHealth

SN - 1612-9202

IS - 3

ER -