Germ-line transmission of transgenes in Xenopus laevis

Nicholas Marsh-Armstrong, Haochu Huang, Deborah L. Berry, Donald D. Brown

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Adult Xenopus laevis frogs made transgenic by restriction enzyme- mediated integration were bred to test the feasibility of establishing lines of frogs that express transgenes. All of the 19 animals raised to sexual maturity generated progeny that expressed the transgene(s). The patterns and levels of expression of green fluorescent protein transgenes driven by a viral promoter, rat promoter, and four X. laevis promoters were all unaffected by passage through the germ line. These results demonstrate the ease of establishing transgenic lines in X. laevis.

Original languageEnglish (US)
Pages (from-to)14389-14393
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number25
DOIs
StatePublished - Dec 7 1999
Externally publishedYes

Fingerprint

Xenopus laevis
Transgenes
Germ Cells
Anura
Green Fluorescent Proteins
Enzymes

Keywords

  • Epigenetic
  • Fluorescence in situ hybridization
  • Gametogenesis
  • Gene regulation
  • Matrix-attachment regions

ASJC Scopus subject areas

  • General

Cite this

Germ-line transmission of transgenes in Xenopus laevis. / Marsh-Armstrong, Nicholas; Huang, Haochu; Berry, Deborah L.; Brown, Donald D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 25, 07.12.1999, p. 14389-14393.

Research output: Contribution to journalArticle

Marsh-Armstrong, Nicholas ; Huang, Haochu ; Berry, Deborah L. ; Brown, Donald D. / Germ-line transmission of transgenes in Xenopus laevis. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 25. pp. 14389-14393.
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