Germ cells are required to maintain a stable sexual phenotype in adult zebrafish

Daniel B. Dranow, Richard P Tucker, Bruce W. Draper

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Sex in zebrafish is not determined by a major chromosomal locus, but instead relies on a mechanism that is influenced by a germ cell-derived signal, as animals that lack germ cells, or specifically oocytes, develop as phenotypic males. These data suggest that during primary sex determination, an oocyte-derived signal acts on the bipotential somatic gonad to promote the female-specific program. However, it is not known if germ cells are required only during the primary sex-determining window, or if they are required throughout adult life to maintain the female sexual phenotype. Here, we show that while wild-type zebrafish do not switch sex as adults, germ cell-depleted adult females readily convert to a male phenotype. Notably, when oocytes are depleted, but germline stem cells remain, adult females sex-revert to sperm-producing males, indicating that a germ cell-derived signal acts on the somatic gonad to promote female development directly or indirectly by repressing male-specific gene expression. These results also confirm that signals from the somatic gonad in turn ensure that the sex appropriate gamete is produced.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalDevelopmental Biology
Volume376
Issue number1
DOIs
StatePublished - Apr 1 2013

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Zebrafish
Germ Cells
Phenotype
Gonads
Oocytes
Spermatozoa
Gene Expression

Keywords

  • Germ cells
  • Oocyte
  • Sex determination

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Germ cells are required to maintain a stable sexual phenotype in adult zebrafish. / Dranow, Daniel B.; Tucker, Richard P; Draper, Bruce W.

In: Developmental Biology, Vol. 376, No. 1, 01.04.2013, p. 43-50.

Research output: Contribution to journalArticle

Dranow, Daniel B. ; Tucker, Richard P ; Draper, Bruce W. / Germ cells are required to maintain a stable sexual phenotype in adult zebrafish. In: Developmental Biology. 2013 ; Vol. 376, No. 1. pp. 43-50.
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