Geometric response to nerve growth factor is preserved in aged rat sensory neurons: A single-neuron culture study

Takayuki Ito, Gen Sobue, Takeshi Yasuda, Koji Kimata, Terunori Mitsuma, Akira Takahashi

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We previously established a single-neuron culture system to analyze the primary effects of neurotrophic factors and reported that NGF promoted neurite extension in young adult (4- to 6-month-old) rat dorsal root ganglion (DRG) neurons by promoting neurite arborization. In this study, we demonstrated that the effects of NGF on neurite regeneration in DRG neurons was well preserved in aged rats (20- to 24-month-old and 33-month-old). NGF did not increase the percent process-bearing neurons in aged rats, which indicated that neuronal survival was not promoted by NGF, but it significantly enhanced the number of branching points, total neurite length, and soma size in aged neurons. These effects of NGF on neurite geometry tended to be reduced to some extent in aged neurons and the initiation of neurite-outgrowth in aged neurons was also delayed as compared with young adult neurons. NGF-responsive subpopulation of neurons, found in the entire range of neuronal size, were preserved in aged rats. These findings indicate that NGF could play an important role in regeneration of injured DRG neurons of aged animals.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalNeurobiology of Aging
Volume14
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • Aging
  • Nerve growth factor
  • Neurite regeneration
  • Primary sensory neuron
  • Single-neuron culture

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

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