Genomic perspective on the photobiology of Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon

Shiladitya DasSarma, Sean P. Kennedy, Brian Berquist, Wailap Victor Ng, Nitin S. Baliga, John L. Spudich, Mark P. Krebs, Jonathan A Eisen, Carl H. Johnson, Leroy Hood

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Halobacterium species display a variety of responses to light, including phototrophic growth, phototactic behavior, and photoprotective mechanisms. The complete genome sequence of Halobacterium species NRC-1 (Proc Natl Acad Sci USA 97: 12176-12181, 2000), coupled with the availability of a battery of methods for its analysis makes this an ideal model system for studying photobiology among the archaea. Here, we review: (1) the structure of the 2.57 Mbp Halobacterium NRC-1 genome, including a large chromosome, two minichromosomes, and 91 transposable IS elements; (2) the purple membrane regulon, which programs the accumulation of large quantities of the light-driven proton pump, bacteriorhodopsin, and allows for a period of phototrophic growth; (3) components of the sophisticated pathways for color-sensitive phototaxis; (4) the gas vesicle gene cluster, which codes for cell buoyancy organelles; (5) pathways for the production of carotenoid pigments and retinal, (6) processes for the repair of DNA damage; and (7) putative homologs of circadian rhythm regulators. We conclude with a discussion of the power of systems biology for comprehensive understanding of Halobacterium NRC-1 photobiology.

Original languageEnglish (US)
Pages (from-to)3-17
Number of pages15
JournalPhotosynthesis Research
Volume70
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Archaea
  • Bacteriorhodopsin
  • Carotenoids
  • Circadian rhythm
  • DNA repair
  • Gas vesicles
  • Halobacteria
  • Retinal
  • Sensory rhodopsin
  • Systems biology

ASJC Scopus subject areas

  • Plant Science

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    DasSarma, S., Kennedy, S. P., Berquist, B., Ng, W. V., Baliga, N. S., Spudich, J. L., Krebs, M. P., Eisen, J. A., Johnson, C. H., & Hood, L. (2001). Genomic perspective on the photobiology of Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon. Photosynthesis Research, 70(1), 3-17. https://doi.org/10.1023/A:1013879706863