Genomic Organization, Promoter Region Analysis, and Chromosome Localization of the Mouse bcl-x Gene

Didier A M Grillot, Maribel González-García, Daryoush Ekhterae, Linda Duan, Naohiro Inohara, Shigeo Ohta, Michael F Seldin, Gabriel Núñez

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

The bcl-x gene, a bcl-2 family member, is highly regulated during lymphoid development, and its expression modulates apoptosis in lymphoid and other cell populations. Several forms of bcl-x mRNAs with different biologic functions have been described in rodents and humans. In this study, we have determined the organization and promoter region of the mouse bcl-x gene in an effort to understand the molecular basis for the different bcl-x mRNA species identified in tissues. We show that mouse bcl-x maps to the distal mouse chromosome 2 at approximately 89 cM, and exhibits a three-exon structure with an untranslated first exon and a facultative first intron. The coding region of bcl-xL is generated by the juncture of exons II and III through a splicing reaction, whereas bcl-xS is generated by an alternatively utilized donor splice site located within exon II. Analysis of multiple cDNAs and primer extension experiments revealed major transcription initiation sites in brain and thymus within a GC-rich region, with multiple Sp1-binding motifs located upstream of exon I. Another promoter was mapped to a 57-bp region localized upstream of the translation initiation codon by transfection of reporter constructs into FL5.12 and K562 cell lines. The remarkable similarity between the genomic regions of bcl-2 and bcl-x suggests that these genes have evolved from a common ancestral gene or through gene duplication.

Original languageEnglish (US)
Pages (from-to)4750-4757
Number of pages8
JournalJournal of Immunology
Volume158
Issue number10
StatePublished - May 15 1997

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Genetic Promoter Regions
Exons
Chromosomes
Genes
GC Rich Sequence
bcl-2 Genes
Messenger RNA
RNA Splice Sites
Gene Duplication
Chromosomes, Human, Pair 2
Initiator Codon
K562 Cells
Transcription Initiation Site
Introns
Thymus Gland
Transfection
Rodentia
Complementary DNA
Organizations
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Grillot, D. A. M., González-García, M., Ekhterae, D., Duan, L., Inohara, N., Ohta, S., ... Núñez, G. (1997). Genomic Organization, Promoter Region Analysis, and Chromosome Localization of the Mouse bcl-x Gene. Journal of Immunology, 158(10), 4750-4757.

Genomic Organization, Promoter Region Analysis, and Chromosome Localization of the Mouse bcl-x Gene. / Grillot, Didier A M; González-García, Maribel; Ekhterae, Daryoush; Duan, Linda; Inohara, Naohiro; Ohta, Shigeo; Seldin, Michael F; Núñez, Gabriel.

In: Journal of Immunology, Vol. 158, No. 10, 15.05.1997, p. 4750-4757.

Research output: Contribution to journalArticle

Grillot, DAM, González-García, M, Ekhterae, D, Duan, L, Inohara, N, Ohta, S, Seldin, MF & Núñez, G 1997, 'Genomic Organization, Promoter Region Analysis, and Chromosome Localization of the Mouse bcl-x Gene', Journal of Immunology, vol. 158, no. 10, pp. 4750-4757.
Grillot DAM, González-García M, Ekhterae D, Duan L, Inohara N, Ohta S et al. Genomic Organization, Promoter Region Analysis, and Chromosome Localization of the Mouse bcl-x Gene. Journal of Immunology. 1997 May 15;158(10):4750-4757.
Grillot, Didier A M ; González-García, Maribel ; Ekhterae, Daryoush ; Duan, Linda ; Inohara, Naohiro ; Ohta, Shigeo ; Seldin, Michael F ; Núñez, Gabriel. / Genomic Organization, Promoter Region Analysis, and Chromosome Localization of the Mouse bcl-x Gene. In: Journal of Immunology. 1997 ; Vol. 158, No. 10. pp. 4750-4757.
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