Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1

Sanjiv Ghanshani, Michael Pak, John Douglas Mcpherson, Michael Strong, Brent Dethlefs, John J. Wasmuth, Lawrence Salkoff, George A. Gutman, K. George Chandy

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Genomic and cDNA clones encoding a novel Shaw-related potassium channel gene have been isolated from mice and humans. The mouse-Kv3.3 gene encodes a protein of 679 amino acids. Unlike the vertebrate Shaker-related genes that have intronless coding regions, mouse Kv3.3 is encoded by at least two exons separated by 3 kb of intervening sequence. The amino-terminal 212 amino acids are encoded by a single exon, and the hydrophobic core of the protein beginning at the S1 transmembrane segment is contained in a separate exon. Multiple Kv3.3-hybridizing transcripts are visible in the mouse brain, liver, thymus, and heart. Using probes derived from a human genomic clone containing the 3′ exon of human Kv3.3 (KCNC3), we have localized the gene to human chromosome 19. The related gene, human Kv3.4 (KCNC4), was localized to human chromosome 1.

Original languageEnglish (US)
Pages (from-to)190-196
Number of pages7
JournalGenomics
Volume12
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

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Shaw Potassium Channels
Chromosomes, Human, Pair 19
Chromosome Mapping
Chromosomes, Human, Pair 1
Human Chromosomes
Exons
Genes
Clone Cells
Amino Acids
Introns
Thymus Gland
Vertebrates
Proteins
Complementary DNA
Liver
Brain

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1. / Ghanshani, Sanjiv; Pak, Michael; Mcpherson, John Douglas; Strong, Michael; Dethlefs, Brent; Wasmuth, John J.; Salkoff, Lawrence; Gutman, George A.; Chandy, K. George.

In: Genomics, Vol. 12, No. 2, 1992, p. 190-196.

Research output: Contribution to journalArticle

Ghanshani, Sanjiv ; Pak, Michael ; Mcpherson, John Douglas ; Strong, Michael ; Dethlefs, Brent ; Wasmuth, John J. ; Salkoff, Lawrence ; Gutman, George A. ; Chandy, K. George. / Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1. In: Genomics. 1992 ; Vol. 12, No. 2. pp. 190-196.
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AU - Strong, Michael

AU - Dethlefs, Brent

AU - Wasmuth, John J.

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