Genomic interrogation of ancestral Mycobacterium tuberculosis from south India

Sujatha Narayanan, Sebastien Gagneux, Lalitha Hari, Anthony G. Tsolaki, Suganthi Rajasekhar, P. R. Narayanan, Peter M. Small, Susan Holmes, Kathryn DeRiemer

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Mycobacterium tuberculosis is a very important global pathogen. One quarter of the world's TB cases occur in India. The tuberculosis strains isolated from south Indian patients exhibit certain phenotypic characteristics like low virulence in guinea-pigs, resistance to isoniazid, thiophene-2-carboxylic acid hydrazide (TCH) and para-amino salicylic acid (PAS), and enhanced susceptibility to H2O2. Besides this, a large percentage of the isolates harbor only a single copy of IS 6110 which makes these strains distinct. Hence, we have studied the genotypic characteristics of these strains by using advanced techniques like Deletion Micro array, deletion PCR, allelic discrimination RT-PCR using several lineage specific markers and KatG G1388T (non-synonymous) polymorphism along with spoligotyping. The analysis of 1215 tuberculosis patient isolates from south India revealed that 85.2% belonged to the ancestral lineage of M. tuberculosis. Comparative whole-genome hybridization identified six new genomic regions within this lineage that were variably deleted.

Original languageEnglish (US)
Pages (from-to)474-483
Number of pages10
JournalInfection, Genetics and Evolution
Volume8
Issue number4
DOIs
StatePublished - Jul 2008
Externally publishedYes

Keywords

  • Genomic deletion
  • Large sequence polymorphism
  • Lineage
  • Spoligotyping
  • Tuberculosis
  • Vaccine

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Microbiology
  • Infectious Diseases

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  • Cite this

    Narayanan, S., Gagneux, S., Hari, L., Tsolaki, A. G., Rajasekhar, S., Narayanan, P. R., Small, P. M., Holmes, S., & DeRiemer, K. (2008). Genomic interrogation of ancestral Mycobacterium tuberculosis from south India. Infection, Genetics and Evolution, 8(4), 474-483. https://doi.org/10.1016/j.meegid.2007.09.007