Genomic interrogation of ancestral Mycobacterium tuberculosis from south India

Sujatha Narayanan, Sebastien Gagneux, Lalitha Hari, Anthony G. Tsolaki, Suganthi Rajasekhar, P. R. Narayanan, Peter M. Small, Susan Holmes, Kathryn DeRiemer

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Mycobacterium tuberculosis is a very important global pathogen. One quarter of the world's TB cases occur in India. The tuberculosis strains isolated from south Indian patients exhibit certain phenotypic characteristics like low virulence in guinea-pigs, resistance to isoniazid, thiophene-2-carboxylic acid hydrazide (TCH) and para-amino salicylic acid (PAS), and enhanced susceptibility to H2O2. Besides this, a large percentage of the isolates harbor only a single copy of IS 6110 which makes these strains distinct. Hence, we have studied the genotypic characteristics of these strains by using advanced techniques like Deletion Micro array, deletion PCR, allelic discrimination RT-PCR using several lineage specific markers and KatG G1388T (non-synonymous) polymorphism along with spoligotyping. The analysis of 1215 tuberculosis patient isolates from south India revealed that 85.2% belonged to the ancestral lineage of M. tuberculosis. Comparative whole-genome hybridization identified six new genomic regions within this lineage that were variably deleted.

Original languageEnglish (US)
Pages (from-to)474-483
Number of pages10
JournalInfection, Genetics and Evolution
Volume8
Issue number4
DOIs
StatePublished - Jul 2008
Externally publishedYes

Fingerprint

tuberculosis
Mycobacterium tuberculosis
India
genomics
Tuberculosis
Polymerase Chain Reaction
Comparative Genomic Hybridization
Salicylic Acid
Isoniazid
hydrazides
thiophene
isoniazid
Virulence
Guinea Pigs
carboxylic acids
salicylic acid
guinea pigs
virulence
hybridization
carboxylic acid

Keywords

  • Genomic deletion
  • Large sequence polymorphism
  • Lineage
  • Spoligotyping
  • Tuberculosis
  • Vaccine

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Microbiology
  • Infectious Diseases

Cite this

Narayanan, S., Gagneux, S., Hari, L., Tsolaki, A. G., Rajasekhar, S., Narayanan, P. R., ... DeRiemer, K. (2008). Genomic interrogation of ancestral Mycobacterium tuberculosis from south India. Infection, Genetics and Evolution, 8(4), 474-483. https://doi.org/10.1016/j.meegid.2007.09.007

Genomic interrogation of ancestral Mycobacterium tuberculosis from south India. / Narayanan, Sujatha; Gagneux, Sebastien; Hari, Lalitha; Tsolaki, Anthony G.; Rajasekhar, Suganthi; Narayanan, P. R.; Small, Peter M.; Holmes, Susan; DeRiemer, Kathryn.

In: Infection, Genetics and Evolution, Vol. 8, No. 4, 07.2008, p. 474-483.

Research output: Contribution to journalArticle

Narayanan, S, Gagneux, S, Hari, L, Tsolaki, AG, Rajasekhar, S, Narayanan, PR, Small, PM, Holmes, S & DeRiemer, K 2008, 'Genomic interrogation of ancestral Mycobacterium tuberculosis from south India', Infection, Genetics and Evolution, vol. 8, no. 4, pp. 474-483. https://doi.org/10.1016/j.meegid.2007.09.007
Narayanan S, Gagneux S, Hari L, Tsolaki AG, Rajasekhar S, Narayanan PR et al. Genomic interrogation of ancestral Mycobacterium tuberculosis from south India. Infection, Genetics and Evolution. 2008 Jul;8(4):474-483. https://doi.org/10.1016/j.meegid.2007.09.007
Narayanan, Sujatha ; Gagneux, Sebastien ; Hari, Lalitha ; Tsolaki, Anthony G. ; Rajasekhar, Suganthi ; Narayanan, P. R. ; Small, Peter M. ; Holmes, Susan ; DeRiemer, Kathryn. / Genomic interrogation of ancestral Mycobacterium tuberculosis from south India. In: Infection, Genetics and Evolution. 2008 ; Vol. 8, No. 4. pp. 474-483.
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