Genomic epidemiology of Salmonella enterica serotype Enteritidis based on population structure of prevalent lineages

Xiangyu Deng, Prerak T. Desai, Henk C. den Bakker, Matthew Mikoleit, Beth Tolar, Eija Trees, Rene S. Hendriksen, Jonathan G. Frye, Steffen Porwollik, Bart C Weimer, Martin Wiedmann, George M. Weinstock, Patricia I. Fields, Michael McClelland

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Salmonella enterica serotype Enteritidis is one of the most commonly reported causes of human salmonellosis. Its low genetic diversity, measured by fingerprinting methods, has made subtyping a challenge. We used whole-genome sequencing to characterize 125 S. enterica Enteritidis and 3 S. enterica serotype Nitra strains. Single-nucleotide polymorphisms were filtered to identify 4, 887 reliable loci that distinguished all isolates from each other. Our wholegenome single-nucleotide polymorphism typing approach was robust for S. enterica Enteritidis subtyping with combined data for different strains from 2 different sequencing platforms. Five major genetic lineages were recognized, which revealed possible patterns of geographic and epidemiologic distribution. Analyses on the population dynamics and evolutionary history estimated that major lineages emerged during the 17th-18th centuries and diversified during the 1920s and 1950s.

Original languageEnglish (US)
Pages (from-to)1481-1489
Number of pages9
JournalEmerging Infectious Diseases
Volume20
Issue number9
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

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    Deng, X., Desai, P. T., den Bakker, H. C., Mikoleit, M., Tolar, B., Trees, E., Hendriksen, R. S., Frye, J. G., Porwollik, S., Weimer, B. C., Wiedmann, M., Weinstock, G. M., Fields, P. I., & McClelland, M. (2014). Genomic epidemiology of Salmonella enterica serotype Enteritidis based on population structure of prevalent lineages. Emerging Infectious Diseases, 20(9), 1481-1489. https://doi.org/10.3201/eid2009.131095